Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4342387..4343006 Replicon chromosome
Accession NZ_AP025009
Organism Raoultella ornithinolytica strain NUITM-VR1

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag LDO46_RS20535 Protein ID WP_004858783.1
Coordinates 4342788..4343006 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag LDO46_RS20530 Protein ID WP_004858785.1
Coordinates 4342387..4342761 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO46_RS20520 (NUITMVR1_40480) 4337497..4338690 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LDO46_RS20525 (NUITMVR1_40490) 4338713..4341859 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit AcrB -
LDO46_RS20530 (NUITMVR1_40500) 4342387..4342761 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
LDO46_RS20535 (NUITMVR1_40510) 4342788..4343006 + 219 WP_004858783.1 HHA domain-containing protein Toxin
LDO46_RS20540 (NUITMVR1_40520) 4343158..4343724 + 567 WP_004858781.1 maltose O-acetyltransferase -
LDO46_RS20545 4343696..4343830 - 135 WP_223306663.1 hypothetical protein -
LDO46_RS20550 (NUITMVR1_40530) 4343824..4344294 + 471 WP_004858780.1 YlaC family protein -
LDO46_RS20555 (NUITMVR1_40540) 4344269..4345720 - 1452 WP_224050077.1 PLP-dependent aminotransferase family protein -
LDO46_RS20560 (NUITMVR1_40550) 4345824..4346534 + 711 WP_015585015.1 GNAT family protein -
LDO46_RS20565 (NUITMVR1_40560) 4346531..4346671 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LDO46_RS20570 (NUITMVR1_40570) 4346674..4346934 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T39900 WP_004858783.1 NZ_AP025009:4342788-4343006 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39900 NZ_AP025009:4342788-4343006 [Raoultella ornithinolytica]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGACCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT39900 WP_004858785.1 NZ_AP025009:4342387-4342761 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39900 NZ_AP025009:4342387-4342761 [Raoultella ornithinolytica]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGATCCAACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGTCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References