Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 69093..69640 Replicon chromosome
Accession NZ_AP025009
Organism Raoultella ornithinolytica strain NUITM-VR1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag LDO46_RS00320 Protein ID WP_048025274.1
Coordinates 69093..69401 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag LDO46_RS00325 Protein ID WP_004869059.1
Coordinates 69404..69640 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO46_RS00290 (NUITMVR1_00580) 64286..64588 - 303 WP_015585486.1 PTS lactose/cellobiose transporter subunit IIA -
LDO46_RS00295 (NUITMVR1_00590) 64723..66048 - 1326 WP_118860249.1 PTS transporter subunit EIIC -
LDO46_RS00300 (NUITMVR1_00600) 66060..66374 - 315 WP_004869063.1 PTS sugar transporter subunit IIB -
LDO46_RS00305 (NUITMVR1_00610) 66669..67610 + 942 WP_015585487.1 LacI family DNA-binding transcriptional regulator -
LDO46_RS00310 (NUITMVR1_00620) 67691..67966 - 276 WP_160873538.1 YicS family protein -
LDO46_RS00315 (NUITMVR1_00630) 68075..68977 - 903 WP_015585496.1 EamA family transporter -
LDO46_RS00320 (NUITMVR1_00640) 69093..69401 - 309 WP_048025274.1 CcdB family protein Toxin
LDO46_RS00325 (NUITMVR1_00650) 69404..69640 - 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LDO46_RS00330 (NUITMVR1_00660) 69746..71179 - 1434 WP_224050106.1 PTS N-acetylmuramic acid transporter subunit IIBC -
LDO46_RS00335 (NUITMVR1_00670) 71204..72106 - 903 WP_088402359.1 N-acetylmuramic acid 6-phosphate etherase -
LDO46_RS00340 (NUITMVR1_00680) 72271..73434 - 1164 WP_044350982.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T39886 WP_048025274.1 NZ_AP025009:c69401-69093 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T39886 NZ_AP025009:c69401-69093 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT39886 WP_004869059.1 NZ_AP025009:c69640-69404 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT39886 NZ_AP025009:c69640-69404 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References