Detailed information of TA system TA039882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 370731..371259
• Replicon: chromosome
• Accession: NZ_AP024968
• Organism: Vibrio cholerae strain V130003

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0X1KZS6
• Locus tag: M2Z51_RS15925
• Protein ID: WP_000221354.1
• Coordinates: 370972..371259 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: M2Z51_RS15920
• Protein ID: WP_032477085.1
• Coordinates: 370731..370982 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2Z51_RS15880 (V130003_30760)	366084..366572	+	489	WP_114717217.1	dual specificity protein phosphatase family protein	-
M2Z51_RS15890 (V130003_30770)	366747..367619	+	873	WP_000254717.1	DUF3800 domain-containing protein	-
M2Z51_RS15895 (V130003_30780)	367770..368093	+	324	WP_000248693.1	DUF4144 domain-containing protein	-
M2Z51_RS15900 (V130003_30790)	368241..368714	+	474	WP_153734401.1	hypothetical protein	-
M2Z51_RS15905 (V130003_30810)	368757..369934	+	1178	WP_086010043.1	IS3 family transposase	-
M2Z51_RS15910 (V130003_30820)	369943..370164	-	222	Protein_378	GNAT family N-acetyltransferase	-
M2Z51_RS15915 (V130003_30830)	370161..370433	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
M2Z51_RS15920 (V130003_30840)	370731..370982	+	252	WP_032477085.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M2Z51_RS15925 (V130003_30850)	370972..371259	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M2Z51_RS15930	371290..371460	-	171	Protein_382	IS30 family transposase	-
M2Z51_RS15935 (V130003_30860)	371778..374564	+	2787	WP_032477086.1	alkaline phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	qnrVC4	-	304895..371259	66364
	-	inside	Integron	-	-	305566..368714	63148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10991.96 Da
• Isoelectric Point: 10.4934

>T39882 WP_000221354.1 NZ_AP024968:370972-371259 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Nucleotide

• Length: 288 bp

>T39882 NZ_AP024968:370972-371259 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTTGGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9150.31 Da
• Isoelectric Point: 4.0334

>AT39882 WP_032477085.1 NZ_AP024968:370731-370982 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGAAIAILNHNKPAAYLVPAETYEYLIDMLEDYELSQIVDSRRADLSQAVEVNI
DDL

Nucleotide

• Length: 252 bp

>AT39882 NZ_AP024968:370731-370982 [Vibrio cholerae]
ATGAGACAAGTGTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACGGCTTTGTTGAATGAGGCTGA
TGGGGCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGAGGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCGGACTTATCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A0X1KZS6