Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-yefM/YoeB-RelB
Location 363601..364111 Replicon chromosome
Accession NZ_AP024968
Organism Vibrio cholerae strain V130003

Toxin (Protein)


Gene name relB Uniprot ID A0A7Z7VK37
Locus tag M2Z51_RS15850 Protein ID WP_000098715.1
Coordinates 363848..364111 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A7Y3QZ95
Locus tag M2Z51_RS15845 Protein ID WP_001223794.1
Coordinates 363601..363855 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2Z51_RS15790 358661..358723 + 63 Protein_356 DUF645 family protein -
M2Z51_RS15795 358695..358811 + 117 WP_232510684.1 Tfp pilus assembly protein -
M2Z51_RS19005 359167..359320 + 154 Protein_358 DUF645 family protein -
M2Z51_RS15810 359677..359840 + 164 Protein_359 DUF645 family protein -
M2Z51_RS15815 (V130003_30680) 360096..360326 - 231 WP_000838533.1 PAS factor family protein -
M2Z51_RS15820 360464..360526 + 63 Protein_361 acetyltransferase -
M2Z51_RS15825 (V130003_30690) 360759..361886 + 1128 WP_172779516.1 ISAs1-like element IS1358 family transposase -
M2Z51_RS15830 361919..362178 + 260 Protein_363 type II toxin-antitoxin system RelE/ParE family toxin -
M2Z51_RS15835 (V130003_30700) 362165..362479 + 315 WP_000071008.1 DNA-binding transcriptional regulator -
M2Z51_RS15840 (V130003_30710) 362618..363391 + 774 WP_032471917.1 aminoglycoside phosphotransferase family protein -
M2Z51_RS15845 (V130003_30720) 363601..363855 + 255 WP_001223794.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
M2Z51_RS15850 (V130003_30730) 363848..364111 + 264 WP_000098715.1 Txe/YoeB family addiction module toxin Toxin
M2Z51_RS15855 364482..364529 + 48 Protein_368 DUF645 family protein -
M2Z51_RS15860 364534..364623 + 90 WP_229598405.1 Tfp pilus assembly protein -
M2Z51_RS15865 (V130003_30740) 364811..365314 + 504 WP_139062842.1 hypothetical protein -
M2Z51_RS15870 365321..365383 + 63 Protein_371 acetyltransferase -
M2Z51_RS15875 (V130003_30750) 365410..365949 + 540 WP_000884557.1 hydrolase -
M2Z51_RS15880 (V130003_30760) 366084..366572 + 489 WP_114717217.1 dual specificity protein phosphatase family protein -
M2Z51_RS15890 (V130003_30770) 366747..367619 + 873 WP_000254717.1 DUF3800 domain-containing protein -
M2Z51_RS15895 (V130003_30780) 367770..368093 + 324 WP_000248693.1 DUF4144 domain-containing protein -
M2Z51_RS15900 (V130003_30790) 368241..368714 + 474 WP_153734401.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 304895..371259 66364
- inside Integron - - 305566..368714 63148
- flank IS/Tn - - 360759..361886 1127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(8-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10415.75 Da        Isoelectric Point: 7.9816

>T39881 WP_000098715.1 NZ_AP024968:363848-364111 [Vibrio cholerae]
MSSRLLSWTDESWDDYLYWQTQDKKTLKRINKIINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDNAITI
ISCRYHY

Download         Length: 264 bp

>T39881 NZ_AP024968:363848-364111 [Vibrio cholerae]
ATGAGTAGTCGTTTACTGTCATGGACAGATGAGTCCTGGGATGACTACTTGTACTGGCAAACACAAGACAAAAAAACACT
CAAGCGGATCAATAAAATCATCAATGATGTTAAGCGTTCACCGTTTGAAGGCATTGGTAAACCAGAGCCTCTAAAAGAAA
ATCTATCTGGTTTTTGGTCTCGACGGATCGATGATACAAATAGACTGGTTTATGCTGTTGATGATAATGCAATAACGATT
ATTTCGTGTCGCTACCATTATTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9437.64 Da        Isoelectric Point: 5.0481

>AT39881 WP_001223794.1 NZ_AP024968:363601-363855 [Vibrio cholerae]
MRIVSFTEARNSLKAVLDAVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPANAEHLNRSIAQFKAGKVTQRD
LIDE

Download         Length: 255 bp

>AT39881 NZ_AP024968:363601-363855 [Vibrio cholerae]
ATGAGAATCGTTTCTTTTACAGAAGCTAGGAATAGTTTAAAAGCAGTCTTGGATGCCGTTGTGAATGATGCGGATACAAC
CGTCATCACTCGACGTGATTCAGAAGATGCCGTGGTTATGTCGCTGGATTATTACAATAGTCTAATGGAAACCGTACATT
TGCTTCGCTCTCCAGCGAACGCTGAACATCTAAACCGTTCCATTGCCCAGTTCAAAGCGGGAAAAGTTACCCAAAGAGAT
CTGATTGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7VK37

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3QZ95

References