Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 352015..352781 Replicon chromosome
Accession NZ_AP024968
Organism Vibrio cholerae strain V130003

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag M2Z51_RS15720 Protein ID WP_000982260.1
Coordinates 352015..352512 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag M2Z51_RS15725 Protein ID WP_000246253.1
Coordinates 352509..352781 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2Z51_RS15670 (V130003_30520) 347475..347795 + 321 WP_000248687.1 DUF4144 domain-containing protein -
M2Z51_RS15675 (V130003_30530) 347962..348321 + 360 WP_001071517.1 VOC family protein -
M2Z51_RS15680 348305..348463 + 159 WP_248529924.1 DUF3265 domain-containing protein -
M2Z51_RS15685 (V130003_30540) 348460..348846 + 387 WP_029628680.1 MmcQ/YjbR family DNA-binding protein -
M2Z51_RS15690 (V130003_30550) 348988..349347 + 360 WP_001900224.1 hypothetical protein -
M2Z51_RS15695 (V130003_30560) 349497..349793 + 297 WP_001069046.1 hypothetical protein -
M2Z51_RS15700 (V130003_30570) 350479..350766 + 288 WP_032477340.1 type II toxin-antitoxin system RelE/ParE family toxin -
M2Z51_RS15705 (V130003_30580) 350777..351094 + 318 WP_032477342.1 HigA family addiction module antitoxin -
M2Z51_RS15710 (V130003_30590) 351295..351576 + 282 WP_032477343.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
M2Z51_RS15715 (V130003_30600) 351573..351860 + 288 WP_000869999.1 type II toxin-antitoxin system RelE/ParE family toxin -
M2Z51_RS15720 (V130003_30610) 352015..352512 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
M2Z51_RS15725 (V130003_30620) 352509..352781 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
M2Z51_RS15730 353170..353310 + 141 WP_001952202.1 DUF645 family protein -
M2Z51_RS15735 (V130003_30630) 353557..353895 + 339 WP_050503336.1 hypothetical protein -
M2Z51_RS15740 (V130003_30640) 354062..354598 + 537 WP_000469478.1 GNAT family protein -
M2Z51_RS15745 (V130003_30650) 354769..355131 + 363 WP_000391481.1 DUF6232 family protein -
M2Z51_RS15750 (V130003_30660) 355318..355554 - 237 WP_000772125.1 RNA-binding protein -
M2Z51_RS15755 (V130003_30670) 355790..356095 + 306 WP_000064427.1 hypothetical protein -
M2Z51_RS15760 356152..356267 + 116 Protein_350 acetyltransferase -
M2Z51_RS15765 356436..356541 + 106 Protein_351 DUF645 family protein -
M2Z51_RS15770 356661..356876 + 216 WP_080284764.1 DUF3709 domain-containing protein -
M2Z51_RS15775 356888..357106 + 219 Protein_353 DUF3709 domain-containing protein -
M2Z51_RS15780 357534..357713 + 180 WP_001908899.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 304895..371259 66364
- inside Integron - - 305566..368714 63148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T39880 WP_000982260.1 NZ_AP024968:c352512-352015 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T39880 NZ_AP024968:c352512-352015 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATTGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCAGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT39880 WP_000246253.1 NZ_AP024968:c352781-352509 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT39880 NZ_AP024968:c352781-352509 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
PDB 1Y9B
AlphaFold DB A0A0F2IC79

References