Detailed information of TA system TA039849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-StbC
• Location: 1525998..1526658
• Replicon: chromosome
• Accession: NZ_AP024955
• Organism: Paraburkholderia terrae strain KU-64

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: PTKU64_RS06800
• Protein ID: WP_091773941.1
• Coordinates: 1526230..1526658 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Z7B428
• Locus tag: PTKU64_RS06795
• Protein ID: WP_007588356.1
• Coordinates: 1525998..1526243 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PTKU64_RS06770 (PTKU64_13250)	1521590..1522102	-	513	WP_131241777.1	Spy/CpxP family protein refolding chaperone	-
PTKU64_RS06775 (PTKU64_13260)	1522255..1523139	+	885	WP_042314786.1	LysR family transcriptional regulator	-
PTKU64_RS06780 (PTKU64_13270)	1523277..1523741	+	465	WP_229513245.1	Lrp/AsnC family transcriptional regulator	-
PTKU64_RS06785 (PTKU64_13280)	1523948..1525045	+	1098	WP_229513246.1	saccharopine dehydrogenase C-terminal domain-containing protein	-
PTKU64_RS06790 (PTKU64_13290)	1525208..1525828	-	621	WP_229513247.1	ATP-binding protein	-
PTKU64_RS06795 (PTKU64_13300)	1525998..1526243	+	246	WP_007588356.1	DNA-binding protein	Antitoxin
PTKU64_RS06800 (PTKU64_13310)	1526230..1526658	+	429	WP_091773941.1	type II toxin-antitoxin system VapC family toxin	Toxin
PTKU64_RS06805 (PTKU64_13320)	1526748..1527221	+	474	WP_229513248.1	DUF2846 domain-containing protein	-
PTKU64_RS06810 (PTKU64_13330)	1527426..1528403	+	978	WP_042314781.1	magnesium/cobalt transporter CorA	-
PTKU64_RS06815 (PTKU64_13340)	1528446..1529084	-	639	WP_229513249.1	LysE family translocator	-
PTKU64_RS06820 (PTKU64_13350)	1529636..1530592	+	957	WP_042314779.1	sugar ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15750.99 Da
• Isoelectric Point: 8.5621

>T39849 WP_091773941.1 NZ_AP024955:1526230-1526658 [Paraburkholderia terrae]
VYLIDTNVISEMRKSSRANPGVQAFFETAKKGGHRLYLSVVTVGELRRGVDLIRHRGDKFQALRLEGWLDTIMSSFSSQI
LNVGTDVAQTWGHLRVPDPEHSLDKLIAATALIYNLTVVTRNVADFDGTGARVLNPFQLLDA

Nucleotide

• Length: 429 bp

>T39849 NZ_AP024955:1526230-1526658 [Paraburkholderia terrae]
GTGTATTTGATTGATACCAATGTAATCAGCGAAATGCGCAAGAGCAGCCGCGCCAATCCAGGCGTGCAGGCTTTTTTCGA
AACGGCGAAGAAAGGCGGGCATCGCCTGTATCTGTCGGTGGTGACGGTCGGCGAGTTGCGGCGCGGCGTCGACCTGATCC
GGCATCGGGGCGACAAGTTCCAGGCGTTGCGCCTGGAAGGCTGGCTCGACACGATCATGAGTTCGTTCAGCAGCCAGATC
CTGAATGTCGGCACCGACGTCGCGCAGACGTGGGGCCATCTGCGCGTGCCGGACCCCGAGCACTCGCTGGACAAGCTGAT
CGCGGCGACGGCCCTGATCTACAACCTGACTGTCGTGACGCGCAACGTGGCAGACTTCGATGGCACGGGCGCACGGGTGC
TGAATCCGTTCCAGCTTCTTGACGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8938.94 Da
• Isoelectric Point: 4.2208

>AT39849 WP_007588356.1 NZ_AP024955:1525998-1526243 [Paraburkholderia terrae]
MANLLVRNLDDALVQNLREQAAAHGRSVEAEHREILAQALFQPKKLSFAELLMSMPDVGDDADFERRNDVTDDSGGPGVF
D

Nucleotide

• Length: 246 bp

>AT39849 NZ_AP024955:1525998-1526243 [Paraburkholderia terrae]
ATGGCAAATCTTTTGGTCCGAAATCTGGACGATGCGTTGGTGCAGAACCTGCGTGAGCAGGCCGCGGCTCACGGGCGCAG
CGTCGAAGCAGAGCATCGGGAAATCCTGGCGCAGGCGCTCTTTCAGCCCAAGAAGCTCAGCTTTGCGGAATTGCTGATGA
GCATGCCCGACGTCGGCGACGACGCCGACTTCGAGCGCCGCAATGACGTCACTGACGATAGCGGGGGACCGGGTGTATTT
GATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z7B428