Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 168268..169827 Replicon plasmid pKAM339_1
Accession NZ_AP024941
Organism Aeromonas caviae strain KAM339

Toxin (Protein)


Gene name hipA Uniprot ID A0A189PGV3
Locus tag LDN97_RS22980 Protein ID WP_011899352.1
Coordinates 168523..169827 (+) Length 435 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A189PGR7
Locus tag LDN97_RS22975 Protein ID WP_011899353.1
Coordinates 168268..168519 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN97_RS22950 (KAM339_044420) 163623..164171 - 549 WP_011899358.1 hypothetical protein -
LDN97_RS22955 (KAM339_044430) 164655..165485 - 831 WP_139026989.1 hypothetical protein -
LDN97_RS22960 (KAM339_044440) 165497..166138 - 642 WP_011899356.1 hypothetical protein -
LDN97_RS22965 (KAM339_044450) 166487..167017 + 531 WP_011899355.1 hypothetical protein -
LDN97_RS22970 (KAM339_044460) 167020..168024 + 1005 WP_162096743.1 tyrosine-type recombinase/integrase -
LDN97_RS22975 (KAM339_044470) 168268..168519 + 252 WP_011899353.1 XRE family transcriptional regulator Antitoxin
LDN97_RS22980 (KAM339_044480) 168523..169827 + 1305 WP_011899352.1 type II toxin-antitoxin system HipA family toxin Toxin
LDN97_RS22985 (KAM339_044490) 169832..170326 + 495 WP_011899351.1 hypothetical protein -
LDN97_RS22990 (KAM339_044500) 171219..172781 + 1563 WP_041206012.1 IS21-like element ISAs28 family transposase -
LDN97_RS22995 (KAM339_044510) 172805..173560 + 756 WP_021141277.1 IS21-like element ISAs28 family helper ATPase IstB -
LDN97_RS23000 (KAM339_044520) 173603..173953 + 351 WP_223936792.1 hypothetical protein -
LDN97_RS23005 (KAM339_044530) 174228..174746 + 519 WP_219265173.1 Hcp family type VI secretion system effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hcp / vgrG1 1..216538 216538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-105)

Antitoxin

HigA2-like, helix-turn-helix domain

(7-67)


Sequences

Toxin        


Download         Length: 435 a.a.        Molecular weight: 48013.16 Da        Isoelectric Point: 6.6361

>T39845 WP_011899352.1 NZ_AP024941:168523-169827 [Aeromonas caviae]
MRAQQLIVAMNGEVVGTLYRAGNGAMSFQYTPEWVAEPGSRPLSLSLPLGHSRIRGSEVFNFFSNLLPDSEAIIARMQAR
FHVETAHPFDLLASVGRDCVGAIQLYPPGIDIPPVTETLAEPLEDAQIEQLLEGYQVAPLGMAEDVDFRISIAGAQEKTA
LLWHQGRWQRPQGSTPTSHIFKLPIGRIVQNNIDLRDSCENEWLCLLIAKAFGFDVANADLAAFGQKRVLIVERFDRRWS
RNGEWLLRLPQEDFCQAMGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNYSIFIEP
ASSFRMTPIYDVMSAFPIFESGGIAAQKAKMAMALQGKNKQYHFAMIQPRHFISTAVHVGFSPDRAARLMHEMAADTESV
ISAVTAELPANFPEHISNAIFQGLSSQAAKIMRG

Download         Length: 1305 bp

>T39845 NZ_AP024941:168523-169827 [Aeromonas caviae]
ATGAGAGCGCAGCAGCTGATAGTGGCAATGAACGGCGAGGTTGTGGGCACATTGTACCGAGCTGGCAACGGAGCCATGTC
ATTCCAATACACGCCTGAATGGGTCGCAGAACCAGGTTCTCGTCCGCTATCACTGTCACTACCTCTGGGCCATAGCCGGA
TCAGAGGAAGTGAAGTCTTCAATTTTTTTAGCAACCTTCTGCCCGACTCCGAGGCGATCATTGCTAGGATGCAGGCCCGG
TTCCACGTTGAGACAGCACACCCCTTTGATCTGTTGGCTAGTGTCGGCCGAGATTGTGTTGGCGCTATCCAGCTCTACCC
GCCCGGTATCGATATCCCTCCTGTCACGGAGACGCTTGCAGAGCCACTGGAAGACGCACAGATTGAGCAGTTGCTGGAAG
GCTATCAGGTGGCACCACTTGGTATGGCTGAGGATGTAGACTTTCGGATCTCGATTGCCGGTGCCCAAGAAAAGACGGCA
TTGCTTTGGCACCAAGGGCGCTGGCAGCGACCGCAGGGCAGCACCCCGACAAGTCATATCTTCAAGCTTCCCATCGGTCG
GATCGTACAAAACAACATCGACCTCCGAGACAGTTGCGAGAATGAATGGCTCTGCCTGCTGATCGCAAAGGCATTTGGCT
TTGACGTTGCCAATGCCGACTTGGCAGCATTTGGCCAAAAGCGTGTGCTGATCGTAGAGCGGTTCGATAGACGCTGGTCG
AGAAACGGTGAATGGTTGTTGAGGTTGCCTCAGGAAGATTTTTGCCAGGCGATGGGGATTGCCCCGGCTCTGAAATACGA
GTCCCATGGTGGCCCCGGCATTGCTGATGCTATGAAGTTGTTGTTGGGTTCCCGGCTAGCAGCCAAGGATAGAGAACAGT
TCTTCAAATCGCAGATCCTGTTCTGGATGTTGGCGGCGATCGATGGGCACGGAAAGAACTACAGCATCTTCATCGAGCCT
GCGTCGTCGTTCAGGATGACCCCCATCTATGATGTCATGTCAGCCTTTCCGATCTTCGAAAGCGGTGGCATTGCAGCTCA
AAAGGCCAAAATGGCGATGGCACTCCAAGGGAAAAATAAGCAGTACCACTTTGCAATGATACAGCCGCGTCACTTCATCA
GTACCGCTGTTCATGTGGGGTTTTCACCGGATAGAGCTGCTCGACTCATGCACGAGATGGCCGCTGACACCGAATCGGTG
ATCTCGGCTGTAACTGCTGAGTTGCCAGCCAATTTCCCTGAGCACATCAGCAATGCCATTTTCCAAGGGCTATCCAGCCA
GGCTGCAAAGATTATGAGGGGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9290.61 Da        Isoelectric Point: 7.2047

>AT39845 WP_011899353.1 NZ_AP024941:168268-168519 [Aeromonas caviae]
MKVTSPASLASAIRDHRKVSKLTQKDAAALVGIKQATVSDFENRPESTKLETLFKILAALDLELHVVKRGSTLDEEQAWD
REW

Download         Length: 252 bp

>AT39845 NZ_AP024941:168268-168519 [Aeromonas caviae]
ATGAAGGTTACATCTCCAGCCTCTCTGGCTTCTGCTATTCGCGACCATCGCAAGGTCAGTAAACTGACGCAGAAAGATGC
TGCGGCATTGGTTGGGATTAAGCAGGCGACAGTCTCAGATTTTGAGAACCGGCCTGAGTCTACCAAGCTGGAGACGCTAT
TCAAGATCCTTGCTGCACTGGATCTGGAGCTTCATGTGGTTAAGCGGGGCTCTACCCTGGATGAGGAGCAAGCTTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A189PGV3

Antitoxin

Source ID Structure
AlphaFold DB A0A189PGR7

References