Detailed information of TA system TA039843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 53694..54367
• Replicon: plasmid pAK1b
• Accession: NZ_AP024938
• Organism: Thermus thermophilus strain AK1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q53W55
• Locus tag: LDO07_RS10960
• Protein ID: WP_011229190.1
• Coordinates: 53694..53954 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3P4AVG9
• Locus tag: LDO07_RS10965
• Protein ID: WP_008631493.1
• Coordinates: 53975..54367 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO07_RS10935 (TthAK1_21520)	49886..50065	+	180	WP_014511253.1	hypothetical protein	-
LDO07_RS10940 (TthAK1_21530)	50075..51244	+	1170	WP_223966976.1	cytochrome P450	-
LDO07_RS10945 (TthAK1_21540)	51241..51939	+	699	WP_143587276.1	lycopene cyclase domain-containing protein	-
LDO07_RS10950 (TthAK1_21550)	51944..52621	+	678	WP_223966978.1	lysophospholipid acyltransferase family protein	-
LDO07_RS10955 (TthAK1_21560)	52618..53640	+	1023	WP_223966980.1	glycosyltransferase family A protein	-
LDO07_RS10960 (TthAK1_21570)	53694..53954	+	261	WP_011229190.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
LDO07_RS10965 (TthAK1_21580)	53975..54367	+	393	WP_008631493.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
LDO07_RS10970 (TthAK1_21590)	54465..56033	+	1569	WP_223966982.1	phytoene desaturase family protein	-
LDO07_RS10975 (TthAK1_21600)	56030..57028	+	999	WP_223966984.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
LDO07_RS10980 (TthAK1_21610)	57038..57430	+	393	WP_011174492.1	nucleotidyltransferase domain-containing protein	-
LDO07_RS10985 (TthAK1_21620)	57461..58006	-	546	WP_223966987.1	YbgA family protein	-
LDO07_RS10990	57990..58217	-	228	Protein_68	transposase	-
LDO07_RS10995	58798..59166	+	369	Protein_69	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..116199	116199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Antitoxin

• PIN domain (2-121)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9894.40 Da
• Isoelectric Point: 8.5953

>T39843 WP_011229190.1 NZ_AP024938:53694-53954 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>T39843 NZ_AP024938:53694-53954 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14234.65 Da
• Isoelectric Point: 10.6447

>AT39843 WP_008631493.1 NZ_AP024938:53975-54367 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>AT39843 NZ_AP024938:53975-54367 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Structures

Toxin

• AlphaFold DB: Q53W55

Antitoxin

• AlphaFold DB: A0A3P4AVG9