Detailed information of TA system TA039841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 169138..169811
• Replicon: plasmid pAA3-7b
• Accession: NZ_AP024934
• Organism: Thermus thermophilus strain AA3-7

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LDO80_RS11745
• Protein ID: WP_267461290.1
• Coordinates: 169138..169530 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: Q53W55
• Locus tag: LDO80_RS11750
• Protein ID: WP_011229190.1
• Coordinates: 169551..169811 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDO80_RS11725 (TthAA37_23290)	165499..166044	+	546	WP_223966987.1	YbgA family protein	-
LDO80_RS11730 (TthAA37_23300)	166075..166467	-	393	WP_011174492.1	nucleotidyltransferase domain-containing protein	-
LDO80_RS11735 (TthAA37_23310)	166477..167475	-	999	WP_223966984.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
LDO80_RS11740 (TthAA37_23320)	167472..169040	-	1569	WP_223966982.1	phytoene desaturase family protein	-
LDO80_RS11745 (TthAA37_23330)	169138..169530	-	393	WP_267461290.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDO80_RS11750 (TthAA37_23340)	169551..169811	-	261	WP_011229190.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LDO80_RS11755 (TthAA37_23350)	169865..170887	-	1023	WP_143587272.1	glycosyltransferase	-
LDO80_RS11760 (TthAA37_23360)	170884..171561	-	678	WP_224065526.1	lysophospholipid acyltransferase family protein	-
LDO80_RS11765 (TthAA37_23370)	171566..172264	-	699	WP_223969273.1	lycopene cyclase domain-containing protein	-
LDO80_RS11770 (TthAA37_23380)	172261..173430	-	1170	WP_011229191.1	cytochrome P450	-
LDO80_RS11775 (TthAA37_23390)	173440..173619	-	180	WP_014511253.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..213325	213325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14218.60 Da
• Isoelectric Point: 10.6447

>T39841 WP_267461290.1 NZ_AP024934:c169530-169138 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAIPSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>T39841 NZ_AP024934:c169530-169138 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCCCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9894.40 Da
• Isoelectric Point: 8.5953

>AT39841 WP_011229190.1 NZ_AP024934:c169811-169551 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>AT39841 NZ_AP024934:c169811-169551 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q53W55