Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/Phd-LOTUS_5_Limkain_b1
Location 190701..191374 Replicon plasmid pAA2-2b
Accession NZ_AP024930
Organism Thermus thermophilus strain AA2-2

Toxin (Protein)


Gene name vapC Uniprot ID Q53W55
Locus tag LDO10_RS11635 Protein ID WP_011229190.1
Coordinates 190701..190961 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3P4AVG9
Locus tag LDO10_RS11640 Protein ID WP_008631493.1
Coordinates 190982..191374 (+) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO10_RS11615 (TthAA22_22820) 187082..188251 + 1170 WP_011229191.1 cytochrome P450 -
LDO10_RS11620 (TthAA22_22830) 188248..188946 + 699 WP_223969273.1 lycopene cyclase domain-containing protein -
LDO10_RS11625 (TthAA22_22840) 188951..189628 + 678 WP_223969271.1 lysophospholipid acyltransferase family protein -
LDO10_RS11630 (TthAA22_22850) 189625..190647 + 1023 WP_143587272.1 glycosyltransferase -
LDO10_RS11635 (TthAA22_22860) 190701..190961 + 261 WP_011229190.1 type II toxin-antitoxin system Phd/YefM family antitoxin Toxin
LDO10_RS11640 (TthAA22_22870) 190982..191374 + 393 WP_008631493.1 type II toxin-antitoxin system VapC family toxin Antitoxin
LDO10_RS11645 (TthAA22_22880) 191472..193040 + 1569 WP_223966982.1 phytoene desaturase family protein -
LDO10_RS11650 (TthAA22_22890) 193037..194035 + 999 WP_223966984.1 type 2 isopentenyl-diphosphate Delta-isomerase -
LDO10_RS11655 (TthAA22_22900) 194045..194437 + 393 WP_011174492.1 nucleotidyltransferase domain-containing protein -
LDO10_RS11660 (TthAA22_22910) 194468..195013 - 546 WP_223966987.1 YbgA family protein -
LDO10_RS11665 (TthAA22_22920) 194997..196349 - 1353 WP_223971549.1 IS200/IS605 family accessory protein TnpB-related protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..298084 298084


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(4-67)

Antitoxin

PIN domain

(2-121)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9894.40 Da        Isoelectric Point: 8.5953

>T39838 WP_011229190.1 NZ_AP024930:190701-190961 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Download         Length: 261 bp

>T39838 NZ_AP024930:190701-190961 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 14234.65 Da        Isoelectric Point: 10.6447

>AT39838 WP_008631493.1 NZ_AP024930:190982-191374 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Download         Length: 393 bp

>AT39838 NZ_AP024930:190982-191374 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q53W55

Antitoxin

Source ID Structure
AlphaFold DB A0A3P4AVG9

References