Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 204159..204832 Replicon plasmid pAA1-1b
Accession NZ_AP024927
Organism Thermus thermophilus strain AA1-1

Toxin (Protein)


Gene name vapB Uniprot ID A0A3P4AVG9
Locus tag LDO09_RS11775 Protein ID WP_008631493.1
Coordinates 204159..204551 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapC Uniprot ID Q53W55
Locus tag LDO09_RS11780 Protein ID WP_011229190.1
Coordinates 204572..204832 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDO09_RS11755 (TthAA11_23230) 200520..201065 + 546 WP_223966987.1 YbgA family protein -
LDO09_RS11760 (TthAA11_23240) 201096..201488 - 393 WP_011174492.1 nucleotidyltransferase domain-containing protein -
LDO09_RS11765 (TthAA11_23250) 201498..202496 - 999 WP_223969268.1 type 2 isopentenyl-diphosphate Delta-isomerase -
LDO09_RS11770 (TthAA11_23260) 202493..204061 - 1569 WP_223966982.1 phytoene desaturase family protein -
LDO09_RS11775 (TthAA11_23270) 204159..204551 - 393 WP_008631493.1 type II toxin-antitoxin system VapC family toxin Toxin
LDO09_RS11780 (TthAA11_23280) 204572..204832 - 261 WP_011229190.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LDO09_RS11785 (TthAA11_23290) 204886..205908 - 1023 WP_143587272.1 glycosyltransferase -
LDO09_RS11790 (TthAA11_23300) 205905..206582 - 678 WP_223969271.1 lysophospholipid acyltransferase family protein -
LDO09_RS11795 (TthAA11_23310) 206587..207285 - 699 WP_223969273.1 lycopene cyclase domain-containing protein -
LDO09_RS11800 (TthAA11_23320) 207282..208451 - 1170 WP_011229191.1 cytochrome P450 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..227954 227954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(4-67)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14234.65 Da        Isoelectric Point: 10.6447

>T39836 WP_008631493.1 NZ_AP024927:c204551-204159 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Download         Length: 393 bp

>T39836 NZ_AP024927:c204551-204159 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9894.40 Da        Isoelectric Point: 8.5953

>AT39836 WP_011229190.1 NZ_AP024927:c204832-204572 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Download         Length: 261 bp

>AT39836 NZ_AP024927:c204832-204572 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P4AVG9

Antitoxin

Source ID Structure
AlphaFold DB Q53W55

References