Detailed information of TA system TA039824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 1203539..1204887
• Replicon: chromosome
• Accession: NZ_AP024905
• Organism: Vibrio ruber strain LMG 23124

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: OCU42_RS05475
• Protein ID: WP_077333672.1
• Coordinates: 1203539..1204480 (-)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A1R4LDG5
• Locus tag: OCU42_RS05480
• Protein ID: WP_000127617.1
• Coordinates: 1204483..1204887 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCU42_RS05450	1199385..1200533	-	1149	WP_077333680.1	ABC transporter permease subunit	-
OCU42_RS05455	1200598..1201620	-	1023	WP_077333678.1	amino acid ABC transporter substrate-binding protein	-
OCU42_RS05460	1201786..1202685	+	900	WP_077333676.1	LysR family transcriptional regulator	-
OCU42_RS05465	1202905..1203156	+	252	WP_139344003.1	hypothetical protein	-
OCU42_RS05470	1203204..1203530	-	327	Protein_1043	type I restriction-modification system subunit M N-terminal domain-containing protein	-
OCU42_RS05475	1203539..1204480	-	942	WP_077333672.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
OCU42_RS05480	1204483..1204887	-	405	WP_000127617.1	DUF6088 family protein	Antitoxin
OCU42_RS05485	1205149..1205337	-	189	WP_000342585.1	hypothetical protein	-
OCU42_RS05490	1205347..1205730	-	384	WP_031854371.1	hypothetical protein	-
OCU42_RS05495	1205723..1206613	-	891	WP_235862845.1	site-specific integrase	-
OCU42_RS05505	1207846..1208295	-	450	WP_235862853.1	hypothetical protein	-
OCU42_RS05510	1208529..1209848	+	1320	WP_077334406.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1207551..1207814	263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-300)

Antitoxin

• Protein of unknown function DUF6088 (8-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35877.41 Da
• Isoelectric Point: 5.8964

>T39824 WP_077333672.1 NZ_AP024905:c1204480-1203539 [Vibrio ruber]
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLAELEALSVETHKLVFSGGTALAKSDVKILRMSEDVDIKLIPSQEFVA
LSRGKKKALRKALVEQIEARIAQSGHFAIEDKLVRNEYRYVEYELKYPQQFSQAPCLRPIIKLELMETISLHAIEERSIQ
SLVSELYQQSREVNAFACVSIQATLVEKVLSMLRRTMLVKRDSERNDDATLVRHIYDVHCIDIAEKTTSEILAPLFHTVL
QEDIQRFGNQHAEFVEDPVNELNLGLQELESNPLFRQRFDDFVKPMVFNPQPHDFDTCFASFKRIAKSLITTI

Nucleotide

• Length: 942 bp

>T39824 NZ_AP024905:c1204480-1203539 [Vibrio ruber]
ATGAAAAAACTAAAACAACAATTCTTAGAGGTTTCCGATGCTTTGGCACTAGGAAACCCAGCGATCATTGAGAAAGATTA
TTGGGTCGTCGCTTTACTTGCGGAGCTTGAAGCACTCTCAGTTGAAACACACAAACTGGTTTTCTCAGGTGGTACGGCAC
TTGCCAAATCTGATGTCAAAATACTCCGTATGTCAGAAGATGTAGATATTAAACTCATTCCTTCACAGGAGTTTGTCGCC
TTAAGCCGTGGTAAAAAAAAGGCTTTACGGAAAGCATTGGTTGAGCAAATCGAAGCGAGAATCGCACAATCAGGTCACTT
TGCTATCGAAGATAAATTAGTACGTAACGAATATCGTTATGTGGAATATGAGCTTAAATATCCTCAACAATTTAGCCAAG
CTCCTTGCTTGCGTCCAATTATCAAGCTTGAGTTAATGGAAACGATTTCTCTTCATGCGATAGAAGAACGCTCCATACAA
TCGTTGGTTTCAGAACTTTACCAACAATCAAGAGAAGTCAACGCGTTTGCTTGCGTTTCTATTCAGGCAACTTTGGTGGA
AAAAGTTCTTTCTATGCTCCGCCGTACCATGTTGGTCAAGCGTGACTCGGAGCGAAACGACGATGCCACTTTGGTTCGTC
ACATTTACGATGTTCACTGTATCGATATCGCGGAAAAAACTACTAGCGAAATACTCGCACCACTTTTTCACACGGTTTTA
CAAGAAGACATCCAACGTTTTGGCAACCAACATGCCGAGTTTGTCGAAGATCCTGTCAATGAATTGAATCTCGGGTTACA
AGAATTAGAGAGTAATCCCCTCTTTCGACAACGTTTTGATGACTTTGTAAAACCAATGGTATTTAACCCACAACCACACG
ATTTTGATACGTGCTTTGCTTCATTTAAACGAATTGCGAAGTCACTGATAACAACCATTTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15292.58 Da
• Isoelectric Point: 10.3464

>AT39824 WP_000127617.1 NZ_AP024905:c1204887-1204483 [Vibrio ruber]
MTAVECIYQKIKRSRRYVFERKDFDDVASYDQVGRALRQLVKQGDLIKIGYGLYTKAKLNSLTGKPMPTHPGGTDALMRE
ILKMKGVDFEMDSLSWQSLSGQSTQIPSSVQYSWNPKQFNRKLVVGNRVLNSQN

Nucleotide

• Length: 405 bp

>AT39824 NZ_AP024905:c1204887-1204483 [Vibrio ruber]
ATGACAGCCGTTGAATGTATTTATCAAAAGATAAAACGCTCTAGACGTTACGTCTTTGAACGTAAAGACTTTGATGATGT
AGCAAGCTACGACCAGGTTGGACGAGCGCTAAGACAACTAGTTAAACAAGGTGATTTGATTAAGATTGGCTACGGTCTGT
ATACCAAAGCCAAACTCAATAGCCTAACGGGTAAACCTATGCCCACACACCCTGGCGGTACTGATGCTCTGATGCGTGAA
ATTTTGAAGATGAAAGGCGTCGATTTTGAAATGGATAGCCTCTCTTGGCAAAGCCTTTCAGGTCAGAGTACTCAAATCCC
TTCATCCGTCCAGTACTCATGGAACCCAAAGCAATTTAATCGCAAATTAGTCGTAGGTAACAGAGTACTTAACTCACAAA
ATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1R4LDG5