Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1114667..1115288 Replicon chromosome
Accession NZ_AP024847
Organism Serratia marcescens strain 2020-O-9

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag LDN81_RS05300 Protein ID WP_004940313.1
Coordinates 1114667..1114870 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag LDN81_RS05305 Protein ID WP_004940312.1
Coordinates 1114920..1115288 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN81_RS05275 (SMGES_10250) 1110458..1110904 + 447 WP_033653249.1 type II secretion system protein GspM -
LDN81_RS05280 1110941..1111756 + 816 WP_052475390.1 A24 family peptidase -
LDN81_RS05285 (SMGES_10270) 1111753..1112115 - 363 WP_041033673.1 type II secretion system pilot lipoprotein GspS -
LDN81_RS05290 (SMGES_10280) 1112369..1113799 + 1431 WP_041033675.1 N-acetylglucosamine-binding protein GbpA -
LDN81_RS05295 (SMGES_10290) 1113852..1114370 + 519 WP_041033677.1 cytochrome b/b6 domain-containing protein -
LDN81_RS05300 (SMGES_10300) 1114667..1114870 - 204 WP_004940313.1 HHA domain-containing protein Toxin
LDN81_RS05305 (SMGES_10310) 1114920..1115288 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
LDN81_RS05310 (SMGES_10320) 1115447..1115800 - 354 WP_033653245.1 hypothetical protein -
LDN81_RS05315 (SMGES_10330) 1116208..1116921 + 714 WP_033647273.1 ABC transporter ATP-binding protein -
LDN81_RS05320 (SMGES_10340) 1116918..1117775 + 858 WP_033637343.1 metal ABC transporter permease -
LDN81_RS05325 (SMGES_10350) 1117801..1118679 + 879 WP_041033680.1 metal ABC transporter substrate-binding protein -
LDN81_RS05330 (SMGES_10360) 1118786..1118926 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
LDN81_RS05335 (SMGES_10370) 1118939..1119193 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T39746 WP_004940313.1 NZ_AP024847:c1114870-1114667 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T39746 NZ_AP024847:c1114870-1114667 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCGGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT39746 WP_004940312.1 NZ_AP024847:c1115288-1114920 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT39746 NZ_AP024847:c1115288-1114920 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCACCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCAGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References