Detailed information of TA system TA039743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 14749..15350
• Replicon: chromosome
• Accession: NZ_AP024847
• Organism: Serratia marcescens strain 2020-O-9

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LDN81_RS00070
• Protein ID: WP_033636540.1
• Coordinates: 14749..15129 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A086G6A0
• Locus tag: LDN81_RS00075
• Protein ID: WP_004933932.1
• Coordinates: 15129..15350 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN81_RS00045 (SMGES_00090)	10002..11282	+	1281	WP_041037321.1	DUF3748 domain-containing protein	-
LDN81_RS00050 (SMGES_00100)	11313..11660	-	348	WP_033636538.1	YceK/YidQ family lipoprotein	-
LDN81_RS00055 (SMGES_00110)	11971..12384	+	414	WP_004933919.1	small heat shock chaperone IbpA	-
LDN81_RS00060 (SMGES_00120)	12485..12913	+	429	WP_004933922.1	small heat shock chaperone IbpB	-
LDN81_RS00065 (SMGES_00140)	13080..14738	+	1659	WP_041037319.1	putative transporter	-
LDN81_RS00070 (SMGES_00150)	14749..15129	-	381	WP_033636540.1	type II toxin-antitoxin system VapC family toxin	Toxin
LDN81_RS00075 (SMGES_00160)	15129..15350	-	222	WP_004933932.1	ribbon-helix-helix domain-containing protein	Antitoxin
LDN81_RS00080 (SMGES_00170)	15425..16675	-	1251	WP_004933935.1	valine--pyruvate transaminase	-
LDN81_RS00085 (SMGES_00180)	16811..18874	-	2064	WP_041037317.1	alpha-amylase	-
LDN81_RS00090 (SMGES_00190)	19071..19223	+	153	WP_019455508.1	hypothetical protein	-
LDN81_RS00095 (SMGES_00200)	19225..20202	-	978	WP_033636542.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14185.53 Da
• Isoelectric Point: 7.3170

>T39743 WP_033636540.1 NZ_AP024847:c15129-14749 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T39743 NZ_AP024847:c15129-14749 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGTTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAATTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTCGGCACTGACAATGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8593.53 Da
• Isoelectric Point: 4.4791

>AT39743 WP_004933932.1 NZ_AP024847:c15350-15129 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT39743 NZ_AP024847:c15350-15129 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGACGGAGAACCACGTTGAAGATCCCAGCAATGCTTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A086G6A0