Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 63327..63934 Replicon plasmid pAA622-2
Accession NZ_AP024839
Organism Enterococcus faecium strain AA622

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag LDN47_RS16125 Protein ID WP_002321032.1
Coordinates 63327..63677 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag LDN47_RS16130 Protein ID WP_002287514.1
Coordinates 63671..63934 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN47_RS16095 (GVanDAA622_32980) 58906..59256 - 351 WP_002303114.1 hypothetical protein -
LDN47_RS16100 (GVanDAA622_32990) 59249..60574 - 1326 WP_002303115.1 Y-family DNA polymerase -
LDN47_RS16105 (GVanDAA622_33000) 60945..61529 - 585 WP_002299575.1 recombinase family protein -
LDN47_RS16110 (GVanDAA622_33010) 61740..61943 - 204 WP_002299573.1 hypothetical protein -
LDN47_RS16115 (GVanDAA622_33020) 61953..62375 - 423 WP_002288787.1 hypothetical protein -
LDN47_RS16120 (GVanDAA622_33030) 62689..63303 + 615 WP_010729795.1 recombinase family protein -
LDN47_RS16125 (GVanDAA622_33040) 63327..63677 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LDN47_RS16130 (GVanDAA622_33050) 63671..63934 - 264 WP_002287514.1 hypothetical protein Antitoxin
LDN47_RS16135 (GVanDAA622_33070) 64275..64454 - 180 WP_002305761.1 hypothetical protein -
LDN47_RS16140 (GVanDAA622_33080) 64470..64670 - 201 WP_002330566.1 hypothetical protein -
LDN47_RS16145 (GVanDAA622_33090) 64835..65542 - 708 WP_002353391.1 hypothetical protein -
LDN47_RS16150 (GVanDAA622_33100) 65535..65972 - 438 WP_002296237.1 hypothetical protein -
LDN47_RS16155 (GVanDAA622_33110) 65987..66238 - 252 WP_002296238.1 hypothetical protein -
LDN47_RS16160 (GVanDAA622_33120) 66238..66444 - 207 WP_002296239.1 hypothetical protein -
LDN47_RS16165 (GVanDAA622_33130) 66885..67145 - 261 WP_002296240.1 hypothetical protein -
LDN47_RS16170 (GVanDAA622_33140) 67511..67996 - 486 WP_002296241.1 single-stranded DNA-binding protein -
LDN47_RS16175 (GVanDAA622_33150) 67999..68898 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) - 1..71967 71967
- flank IS/Tn tet(M) - 51424..58849 7425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T39740 WP_002321032.1 NZ_AP024839:c63677-63327 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T39740 NZ_AP024839:c63677-63327 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT39740 WP_002287514.1 NZ_AP024839:c63934-63671 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT39740 NZ_AP024839:c63934-63671 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References