Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907-MazE |
| Location | 63327..63934 | Replicon | plasmid pAA622-2 |
| Accession | NZ_AP024839 | ||
| Organism | Enterococcus faecium strain AA622 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | E3USU0 |
| Locus tag | LDN47_RS16125 | Protein ID | WP_002321032.1 |
| Coordinates | 63327..63677 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A829F3C0 |
| Locus tag | LDN47_RS16130 | Protein ID | WP_002287514.1 |
| Coordinates | 63671..63934 (-) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LDN47_RS16095 (GVanDAA622_32980) | 58906..59256 | - | 351 | WP_002303114.1 | hypothetical protein | - |
| LDN47_RS16100 (GVanDAA622_32990) | 59249..60574 | - | 1326 | WP_002303115.1 | Y-family DNA polymerase | - |
| LDN47_RS16105 (GVanDAA622_33000) | 60945..61529 | - | 585 | WP_002299575.1 | recombinase family protein | - |
| LDN47_RS16110 (GVanDAA622_33010) | 61740..61943 | - | 204 | WP_002299573.1 | hypothetical protein | - |
| LDN47_RS16115 (GVanDAA622_33020) | 61953..62375 | - | 423 | WP_002288787.1 | hypothetical protein | - |
| LDN47_RS16120 (GVanDAA622_33030) | 62689..63303 | + | 615 | WP_010729795.1 | recombinase family protein | - |
| LDN47_RS16125 (GVanDAA622_33040) | 63327..63677 | - | 351 | WP_002321032.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LDN47_RS16130 (GVanDAA622_33050) | 63671..63934 | - | 264 | WP_002287514.1 | hypothetical protein | Antitoxin |
| LDN47_RS16135 (GVanDAA622_33070) | 64275..64454 | - | 180 | WP_002305761.1 | hypothetical protein | - |
| LDN47_RS16140 (GVanDAA622_33080) | 64470..64670 | - | 201 | WP_002330566.1 | hypothetical protein | - |
| LDN47_RS16145 (GVanDAA622_33090) | 64835..65542 | - | 708 | WP_002353391.1 | hypothetical protein | - |
| LDN47_RS16150 (GVanDAA622_33100) | 65535..65972 | - | 438 | WP_002296237.1 | hypothetical protein | - |
| LDN47_RS16155 (GVanDAA622_33110) | 65987..66238 | - | 252 | WP_002296238.1 | hypothetical protein | - |
| LDN47_RS16160 (GVanDAA622_33120) | 66238..66444 | - | 207 | WP_002296239.1 | hypothetical protein | - |
| LDN47_RS16165 (GVanDAA622_33130) | 66885..67145 | - | 261 | WP_002296240.1 | hypothetical protein | - |
| LDN47_RS16170 (GVanDAA622_33140) | 67511..67996 | - | 486 | WP_002296241.1 | single-stranded DNA-binding protein | - |
| LDN47_RS16175 (GVanDAA622_33150) | 67999..68898 | - | 900 | WP_002322787.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(M) | - | 1..71967 | 71967 | |
| - | flank | IS/Tn | tet(M) | - | 51424..58849 | 7425 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13255.28 Da Isoelectric Point: 8.0280
>T39740 WP_002321032.1 NZ_AP024839:c63677-63327 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK
Download Length: 351 bp
>T39740 NZ_AP024839:c63677-63327 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA
Antitoxin
Download Length: 88 a.a. Molecular weight: 10183.60 Da Isoelectric Point: 4.6865
>AT39740 WP_002287514.1 NZ_AP024839:c63934-63671 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW
Download Length: 264 bp
>AT39740 NZ_AP024839:c63934-63671 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F5H8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F3C0 |