Detailed information of TA system TA039738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 554285..554856
• Replicon: chromosome
• Accession: NZ_AP024837
• Organism: Enterococcus faecium strain AA622

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3Y2B8
• Locus tag: LDN47_RS02650
• Protein ID: WP_002286801.1
• Coordinates: 554515..554856 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: LDN47_RS02645
• Protein ID: WP_002323011.1
• Coordinates: 554285..554515 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LDN47_RS02620 (GVanDAA622_05430)	549766..551094	+	1329	WP_002327434.1	FAD-containing oxidoreductase	-
LDN47_RS02625 (GVanDAA622_05440)	551116..551742	+	627	WP_002352507.1	cysteine hydrolase	-
LDN47_RS02630 (GVanDAA622_05450)	551925..552506	+	582	WP_002327433.1	TetR/AcrR family transcriptional regulator	-
LDN47_RS02635 (GVanDAA622_05460)	552871..553446	+	576	WP_002352505.1	SOS response-associated peptidase family protein	-
LDN47_RS02640 (GVanDAA622_05470)	553651..553989	-	339	WP_002306002.1	hypothetical protein	-
LDN47_RS02645 (GVanDAA622_05480)	554285..554515	+	231	WP_002323011.1	hypothetical protein	Antitoxin
LDN47_RS02650 (GVanDAA622_05490)	554515..554856	+	342	WP_002286801.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LDN47_RS02655	555191..555648	+	458	Protein_525	transposase	-
LDN47_RS02660 (GVanDAA622_05530)	555743..556701	+	959	Protein_526	IS30 family transposase	-
LDN47_RS02665 (GVanDAA622_05540)	556707..557417	+	711	Protein_527	IS3-like element IS1485 family transposase	-
LDN47_RS02670	557548..557790	+	243	Protein_528	LPXTG cell wall anchor domain-containing protein	-
LDN47_RS02675 (GVanDAA622_05560)	557864..558760	+	897	WP_002352497.1	class C sortase	-
LDN47_RS02680 (GVanDAA622_05570)	558941..559615	+	675	WP_002291233.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	552871..559615	6744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13302.68 Da
• Isoelectric Point: 9.9044

>T39738 WP_002286801.1 NZ_AP024837:554515-554856 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T39738 NZ_AP024837:554515-554856 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT39738 WP_002323011.1 NZ_AP024837:554285-554515 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT39738 NZ_AP024837:554285-554515 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A829FD66

Antitoxin

• AlphaFold DB: A0A828ZZN9