Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 222437..222966 Replicon chromosome
Accession NZ_AP024837
Organism Enterococcus faecium strain AA622

Toxin (Protein)


Gene name relE Uniprot ID Q3XX67
Locus tag LDN47_RS01100 Protein ID WP_002296560.1
Coordinates 222700..222966 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A829F3T0
Locus tag LDN47_RS01095 Protein ID WP_002296559.1
Coordinates 222437..222700 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN47_RS01085 (GVanDAA622_02220) 219811..221634 + 1824 WP_002294202.1 APC family permease -
LDN47_RS01090 (GVanDAA622_02230) 221776..221961 - 186 WP_002296558.1 transcriptional regulator -
LDN47_RS01095 (GVanDAA622_02240) 222437..222700 + 264 WP_002296559.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LDN47_RS01100 (GVanDAA622_02250) 222700..222966 + 267 WP_002296560.1 Txe/YoeB family addiction module toxin Toxin
LDN47_RS01105 (GVanDAA622_02260) 223167..223655 + 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
LDN47_RS01110 (GVanDAA622_02270) 224196..224849 - 654 WP_002323018.1 ABC transporter permease -
LDN47_RS01115 (GVanDAA622_02280) 224864..225781 - 918 WP_002296564.1 osmoprotectant ABC transporter substrate-binding protein -
LDN47_RS01120 (GVanDAA622_02290) 225778..226419 - 642 WP_002293405.1 ABC transporter permease -
LDN47_RS01125 (GVanDAA622_02300) 226423..227004 - 582 Protein_223 CBS domain-containing protein -
LDN47_RS01130 (GVanDAA622_02310) 227162..227566 + 405 WP_002287522.1 IS200/IS605-like element ISEfa4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10660.13 Da        Isoelectric Point: 9.9931

>T39737 WP_002296560.1 NZ_AP024837:222700-222966 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T39737 NZ_AP024837:222700-222966 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10046.05 Da        Isoelectric Point: 4.1207

>AT39737 WP_002296559.1 NZ_AP024837:222437-222700 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNID
DIDWEAL

Download         Length: 264 bp

>AT39737 NZ_AP024837:222437-222700 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FCW8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3T0

References