Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 12866..13473 Replicon plasmid pAA620-2
Accession NZ_AP024833
Organism Enterococcus faecium strain AA620

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag LDN34_RS15300 Protein ID WP_002321032.1
Coordinates 12866..13216 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag LDN34_RS15305 Protein ID WP_002287514.1
Coordinates 13210..13473 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN34_RS15270 (GVanDAA620_31030) 8445..8795 - 351 WP_002303114.1 hypothetical protein -
LDN34_RS15275 (GVanDAA620_31040) 8788..10113 - 1326 WP_002303115.1 Y-family DNA polymerase -
LDN34_RS15280 (GVanDAA620_31050) 10484..11068 - 585 WP_002299575.1 recombinase family protein -
LDN34_RS15285 (GVanDAA620_31060) 11279..11482 - 204 WP_002299573.1 hypothetical protein -
LDN34_RS15290 (GVanDAA620_31070) 11492..11914 - 423 WP_002288787.1 hypothetical protein -
LDN34_RS15295 (GVanDAA620_31080) 12228..12842 + 615 WP_010729795.1 recombinase family protein -
LDN34_RS15300 (GVanDAA620_31090) 12866..13216 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LDN34_RS15305 (GVanDAA620_31100) 13210..13473 - 264 WP_002287514.1 hypothetical protein Antitoxin
LDN34_RS15310 (GVanDAA620_31120) 13814..13993 - 180 WP_002305761.1 hypothetical protein -
LDN34_RS15315 (GVanDAA620_31130) 14009..14209 - 201 WP_002330566.1 hypothetical protein -
LDN34_RS15320 (GVanDAA620_31140) 14374..15081 - 708 WP_002353391.1 hypothetical protein -
LDN34_RS15325 (GVanDAA620_31150) 15074..15511 - 438 WP_002296237.1 hypothetical protein -
LDN34_RS15330 (GVanDAA620_31160) 15526..15777 - 252 WP_002296238.1 hypothetical protein -
LDN34_RS15335 (GVanDAA620_31170) 15777..15983 - 207 WP_002296239.1 hypothetical protein -
LDN34_RS15340 (GVanDAA620_31180) 16424..16684 - 261 WP_002296240.1 hypothetical protein -
LDN34_RS15345 (GVanDAA620_31190) 17050..17535 - 486 WP_002296241.1 single-stranded DNA-binding protein -
LDN34_RS15350 (GVanDAA620_31200) 17538..18437 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) - 1..71966 71966
- flank IS/Tn tet(M) - 963..8388 7425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T39736 WP_002321032.1 NZ_AP024833:c13216-12866 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T39736 NZ_AP024833:c13216-12866 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT39736 WP_002287514.1 NZ_AP024833:c13473-13210 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT39736 NZ_AP024833:c13473-13210 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References