Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/PRK09907-MazE |
| Location | 12866..13473 | Replicon | plasmid pAA620-2 |
| Accession | NZ_AP024833 | ||
| Organism | Enterococcus faecium strain AA620 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | E3USU0 |
| Locus tag | LDN34_RS15300 | Protein ID | WP_002321032.1 |
| Coordinates | 12866..13216 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A829F3C0 |
| Locus tag | LDN34_RS15305 | Protein ID | WP_002287514.1 |
| Coordinates | 13210..13473 (-) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LDN34_RS15270 (GVanDAA620_31030) | 8445..8795 | - | 351 | WP_002303114.1 | hypothetical protein | - |
| LDN34_RS15275 (GVanDAA620_31040) | 8788..10113 | - | 1326 | WP_002303115.1 | Y-family DNA polymerase | - |
| LDN34_RS15280 (GVanDAA620_31050) | 10484..11068 | - | 585 | WP_002299575.1 | recombinase family protein | - |
| LDN34_RS15285 (GVanDAA620_31060) | 11279..11482 | - | 204 | WP_002299573.1 | hypothetical protein | - |
| LDN34_RS15290 (GVanDAA620_31070) | 11492..11914 | - | 423 | WP_002288787.1 | hypothetical protein | - |
| LDN34_RS15295 (GVanDAA620_31080) | 12228..12842 | + | 615 | WP_010729795.1 | recombinase family protein | - |
| LDN34_RS15300 (GVanDAA620_31090) | 12866..13216 | - | 351 | WP_002321032.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| LDN34_RS15305 (GVanDAA620_31100) | 13210..13473 | - | 264 | WP_002287514.1 | hypothetical protein | Antitoxin |
| LDN34_RS15310 (GVanDAA620_31120) | 13814..13993 | - | 180 | WP_002305761.1 | hypothetical protein | - |
| LDN34_RS15315 (GVanDAA620_31130) | 14009..14209 | - | 201 | WP_002330566.1 | hypothetical protein | - |
| LDN34_RS15320 (GVanDAA620_31140) | 14374..15081 | - | 708 | WP_002353391.1 | hypothetical protein | - |
| LDN34_RS15325 (GVanDAA620_31150) | 15074..15511 | - | 438 | WP_002296237.1 | hypothetical protein | - |
| LDN34_RS15330 (GVanDAA620_31160) | 15526..15777 | - | 252 | WP_002296238.1 | hypothetical protein | - |
| LDN34_RS15335 (GVanDAA620_31170) | 15777..15983 | - | 207 | WP_002296239.1 | hypothetical protein | - |
| LDN34_RS15340 (GVanDAA620_31180) | 16424..16684 | - | 261 | WP_002296240.1 | hypothetical protein | - |
| LDN34_RS15345 (GVanDAA620_31190) | 17050..17535 | - | 486 | WP_002296241.1 | single-stranded DNA-binding protein | - |
| LDN34_RS15350 (GVanDAA620_31200) | 17538..18437 | - | 900 | WP_002322787.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(M) | - | 1..71966 | 71966 | |
| - | flank | IS/Tn | tet(M) | - | 963..8388 | 7425 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13255.28 Da Isoelectric Point: 8.0280
>T39736 WP_002321032.1 NZ_AP024833:c13216-12866 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK
Download Length: 351 bp
>T39736 NZ_AP024833:c13216-12866 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA
Antitoxin
Download Length: 88 a.a. Molecular weight: 10183.60 Da Isoelectric Point: 4.6865
>AT39736 WP_002287514.1 NZ_AP024833:c13473-13210 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW
Download Length: 264 bp
>AT39736 NZ_AP024833:c13473-13210 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F5H8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F3C0 |