Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 554277..554848 Replicon chromosome
Accession NZ_AP024831
Organism Enterococcus faecium strain AA620

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LDN34_RS02655 Protein ID WP_002286801.1
Coordinates 554507..554848 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LDN34_RS02650 Protein ID WP_002323011.1
Coordinates 554277..554507 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LDN34_RS02625 (GVanDAA620_05420) 549758..551086 + 1329 WP_002327434.1 FAD-containing oxidoreductase -
LDN34_RS02630 (GVanDAA620_05430) 551108..551734 + 627 WP_002352507.1 cysteine hydrolase -
LDN34_RS02635 (GVanDAA620_05440) 551917..552498 + 582 WP_002327433.1 TetR/AcrR family transcriptional regulator -
LDN34_RS02640 (GVanDAA620_05450) 552863..553438 + 576 WP_002352505.1 SOS response-associated peptidase family protein -
LDN34_RS02645 (GVanDAA620_05460) 553643..553981 - 339 WP_002306002.1 hypothetical protein -
LDN34_RS02650 (GVanDAA620_05470) 554277..554507 + 231 WP_002323011.1 hypothetical protein Antitoxin
LDN34_RS02655 (GVanDAA620_05480) 554507..554848 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LDN34_RS02660 555183..555640 + 458 Protein_525 transposase -
LDN34_RS02665 555735..556694 + 960 Protein_526 IS30 family transposase -
LDN34_RS02670 556700..557407 + 708 Protein_527 IS3 family transposase -
LDN34_RS02675 557538..557780 + 243 Protein_528 LPXTG cell wall anchor domain-containing protein -
LDN34_RS02680 (GVanDAA620_05560) 557854..558750 + 897 WP_002352497.1 class C sortase -
LDN34_RS02685 (GVanDAA620_05570) 558931..559605 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 552863..559605 6742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T39735 WP_002286801.1 NZ_AP024831:554507-554848 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T39735 NZ_AP024831:554507-554848 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT39735 WP_002323011.1 NZ_AP024831:554277-554507 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT39735 NZ_AP024831:554277-554507 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References