Detailed information of TA system TA039727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4688..5273
• Replicon: plasmid pOCUAc17-3
• Accession: NZ_AP024801
• Organism: Acinetobacter pittii strain OCU Ac17

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A158LPL9
• Locus tag: OCUAc17_RS19635
• Protein ID: WP_002124950.1
• Coordinates: 4986..5273 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A062GCE4
• Locus tag: OCUAc17_RS19630
• Protein ID: WP_005065064.1
• Coordinates: 4688..4999 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAc17_RS19595 (OCUAc17_38230)	1..930	+	930	WP_005065049.1	replication initiation protein RepM	-
OCUAc17_RS19600 (OCUAc17_38240)	1009..1539	+	531	WP_005065050.1	plasmid replication DNA-binding protein	-
OCUAc17_RS19605 (OCUAc17_38250)	1889..2206	+	318	WP_000900860.1	hypothetical protein	-
OCUAc17_RS19610 (OCUAc17_38260)	2727..3071	+	345	WP_005065055.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OCUAc17_RS19615 (OCUAc17_38270)	3076..3378	+	303	WP_005065057.1	XRE family transcriptional regulator	-
OCUAc17_RS19620 (OCUAc17_38280)	3470..3871	+	402	WP_005065059.1	hypothetical protein	-
OCUAc17_RS19625 (OCUAc17_38290)	3923..4597	+	675	WP_005065062.1	HEPN domain-containing protein	-
OCUAc17_RS19630 (OCUAc17_38300)	4688..4999	-	312	WP_005065064.1	BrnA antitoxin family protein	Antitoxin
OCUAc17_RS19635 (OCUAc17_38310)	4986..5273	-	288	WP_002124950.1	BrnT family toxin	Toxin
OCUAc17_RS19640 (OCUAc17_38320)	5786..7273	+	1488	WP_005065066.1	SulP family inorganic anion transporter	-
OCUAc17_RS19645 (OCUAc17_38330)	7286..8137	+	852	WP_005065068.1	universal stress protein	-
OCUAc17_RS19650 (OCUAc17_38340)	8408..9292	-	885	WP_005065070.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11158	11158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11173.58 Da
• Isoelectric Point: 4.9093

>T39727 WP_002124950.1 NZ_AP024801:c5273-4986 [Acinetobacter pittii]
MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNGEERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T39727 NZ_AP024801:c5273-4986 [Acinetobacter pittii]
ATGGAACTGTATTTCGAATGGGATGAGGCTAAAAACCAAAAGAATCAGAAAAAACATGATGTATCTTTTGAAACGGCAAG
CCTCGTTTTTGAAGATCCTTTACGGATCTCAATCCAGGATCGACATACCAATGGTGAAGAACGTTGGCAAACGATCGGTA
TGGTTAAAGGTGTACTGATGTTATTGGTGGCTCATACAATCATTGACGATGATGACTGTGAGATCATACGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAACGCAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 12070.80 Da
• Isoelectric Point: 9.4651

>AT39727 WP_005065064.1 NZ_AP024801:c4999-4688 [Acinetobacter pittii]
MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEITDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLEDLKNH

Nucleotide

• Length: 312 bp

>AT39727 NZ_AP024801:c4999-4688 [Acinetobacter pittii]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGAAAAATTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCAAAAGGAACTGTACCAGATGAACAATTAGATCTATCTGACATTCCTGAGATTACCGACTGGAGTCATGCTGTTCGCC
ATGGGCAATTTTATCGTCCAGTGAAGCAACAAACATCTGTTCGTCTAGATGCAGATGTACTTGCATGGTTAAAGGCTCAA
GGCAAAGGCTATCAGACACGCATGAATAAAATTCTGCGTGAAGCAATGCTAGAAGATCTTAAGAATCATTGA

Structures

Toxin

• AlphaFold DB: A0A158LPL9

Antitoxin

• AlphaFold DB: A0A062GCE4