Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61900..62543 Replicon plasmid IncN-R
Accession NZ_AP024796
Organism Klebsiella pneumoniae strain TUM12140

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6J94_RS26715 Protein ID WP_001044770.1
Coordinates 62127..62543 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6J94_RS26710 Protein ID WP_001261282.1
Coordinates 61900..62130 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J94_RS27390 (TUM12140_51280) 59184..59363 + 180 Protein_65 hypothetical protein -
K6J94_RS26690 (TUM12140_51290) 59421..60197 - 777 WP_000015958.1 site-specific integrase -
K6J94_RS26695 (TUM12140_51300) 60194..60937 - 744 WP_000129823.1 hypothetical protein -
K6J94_RS26700 (TUM12140_51310) 60988..61338 - 351 WP_000493378.1 hypothetical protein -
K6J94_RS26705 61482..61943 - 462 WP_072202616.1 hypothetical protein -
K6J94_RS26710 (TUM12140_51320) 61900..62130 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6J94_RS26715 (TUM12140_51330) 62127..62543 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J94_RS26720 (TUM12140_51340) 62617..63306 + 690 Protein_72 AAA family ATPase -
K6J94_RS26725 63361..64058 + 698 WP_223174630.1 IS1 family transposase -
K6J94_RS26730 64074..64184 + 111 Protein_74 mercuric transport protein periplasmic component -
K6J94_RS26735 (TUM12140_51370) 64220..64642 + 423 WP_001340589.1 organomercurial transporter MerC -
K6J94_RS26740 (TUM12140_51380) 64694..66388 + 1695 WP_000105636.1 mercury(II) reductase -
K6J94_RS26745 (TUM12140_51390) 66406..66768 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6J94_RS26750 (TUM12140_51400) 66765..67001 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / aac(6')-Ib / blaTEM-1A / blaOXA-9 / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..133781 133781
- inside IScluster/Tn aac(6')-Ib - 53582..64058 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39719 WP_001044770.1 NZ_AP024796:62127-62543 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39719 NZ_AP024796:62127-62543 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39719 WP_001261282.1 NZ_AP024796:61900-62130 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39719 NZ_AP024796:61900-62130 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References