Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5275189..5275814 Replicon chromosome
Accession NZ_AP024795
Organism Klebsiella pneumoniae strain TUM12140

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6J94_RS26010 Protein ID WP_002882817.1
Coordinates 5275189..5275572 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6J94_RS26015 Protein ID WP_004150355.1
Coordinates 5275572..5275814 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J94_RS25995 (TUM12140_49960) 5272555..5273457 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6J94_RS26000 (TUM12140_49970) 5273454..5274089 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6J94_RS26005 (TUM12140_49980) 5274086..5275015 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6J94_RS26010 (TUM12140_49990) 5275189..5275572 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J94_RS26015 (TUM12140_50000) 5275572..5275814 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6J94_RS26020 (TUM12140_50010) 5276019..5276936 + 918 WP_002882812.1 alpha/beta hydrolase -
K6J94_RS26025 (TUM12140_50020) 5276950..5277891 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6J94_RS26030 (TUM12140_50030) 5277936..5278373 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6J94_RS26035 (TUM12140_50040) 5278370..5279230 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6J94_RS26040 (TUM12140_50050) 5279224..5279823 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T39718 WP_002882817.1 NZ_AP024795:c5275572-5275189 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T39718 NZ_AP024795:c5275572-5275189 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT39718 WP_004150355.1 NZ_AP024795:c5275814-5275572 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT39718 NZ_AP024795:c5275814-5275572 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References