Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4075789..4076408 Replicon chromosome
Accession NZ_AP024795
Organism Klebsiella pneumoniae strain TUM12140

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J94_RS20255 Protein ID WP_002892050.1
Coordinates 4076190..4076408 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J94_RS20250 Protein ID WP_002892066.1
Coordinates 4075789..4076163 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J94_RS20240 (TUM12140_38950) 4070941..4072134 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6J94_RS20245 (TUM12140_38960) 4072157..4075303 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J94_RS20250 (TUM12140_38970) 4075789..4076163 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J94_RS20255 (TUM12140_38980) 4076190..4076408 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J94_RS20260 (TUM12140_38990) 4076567..4077133 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6J94_RS20265 4077105..4077245 - 141 WP_004147370.1 hypothetical protein -
K6J94_RS20270 (TUM12140_39000) 4077266..4077736 + 471 WP_002892026.1 YlaC family protein -
K6J94_RS20275 (TUM12140_39010) 4077711..4079162 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J94_RS20280 (TUM12140_39020) 4079263..4079961 + 699 WP_002892021.1 GNAT family protein -
K6J94_RS20285 (TUM12140_39030) 4079958..4080098 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J94_RS20290 (TUM12140_39040) 4080098..4080361 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39714 WP_002892050.1 NZ_AP024795:4076190-4076408 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39714 NZ_AP024795:4076190-4076408 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39714 WP_002892066.1 NZ_AP024795:4075789-4076163 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39714 NZ_AP024795:4075789-4076163 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References