Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60348..60991 Replicon plasmid IncN-R
Accession NZ_AP024793
Organism Klebsiella pneumoniae strain TUM12139

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6J92_RS26700 Protein ID WP_001044770.1
Coordinates 60348..60764 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6J92_RS26705 Protein ID WP_001261282.1
Coordinates 60761..60991 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J92_RS26665 (TUM12139_51270) 55890..56126 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
K6J92_RS26670 (TUM12139_51280) 56123..56485 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6J92_RS26675 (TUM12139_51290) 56503..58197 - 1695 WP_000105636.1 mercury(II) reductase -
K6J92_RS26680 (TUM12139_51300) 58249..58671 - 423 WP_001340589.1 organomercurial transporter MerC -
K6J92_RS26685 58707..58817 - 111 Protein_65 mercuric transport protein periplasmic component -
K6J92_RS26695 (TUM12139_51330) 59585..60274 - 690 Protein_67 AAA family ATPase -
K6J92_RS26700 (TUM12139_51340) 60348..60764 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J92_RS26705 (TUM12139_51350) 60761..60991 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6J92_RS26710 60948..61409 + 462 WP_072202616.1 hypothetical protein -
K6J92_RS26715 (TUM12139_51360) 61553..61903 + 351 WP_000493378.1 hypothetical protein -
K6J92_RS26720 (TUM12139_51370) 61954..62697 + 744 WP_000129823.1 hypothetical protein -
K6J92_RS26725 (TUM12139_51380) 62694..63470 + 777 WP_000015958.1 site-specific integrase -
K6J92_RS27375 (TUM12139_51390) 63528..63707 - 180 Protein_74 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..133170 133170
- inside IScluster/Tn aac(6')-Ib - 58833..69309 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39700 WP_001044770.1 NZ_AP024793:c60764-60348 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39700 NZ_AP024793:c60764-60348 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39700 WP_001261282.1 NZ_AP024793:c60991-60761 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39700 NZ_AP024793:c60991-60761 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References