Detailed information of TA system TA039681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 60865..61508
• Replicon: plasmid IncN-R
• Accession: NZ_AP024790
• Organism: Klebsiella pneumoniae strain TUM12138

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: K6K06_RS27415
• Protein ID: WP_001044770.1
• Coordinates: 60865..61281 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: K6K06_RS27420
• Protein ID: WP_001261282.1
• Coordinates: 61278..61508 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6K06_RS27380 (TUM12138_52870)	56407..56643	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
K6K06_RS27385 (TUM12138_52880)	56640..57002	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
K6K06_RS27390 (TUM12138_52890)	57020..58714	-	1695	WP_000105636.1	mercury(II) reductase	-
K6K06_RS27395 (TUM12138_52900)	58766..59188	-	423	WP_001340589.1	organomercurial transporter MerC	-
K6K06_RS27400	59224..59334	-	111	Protein_65	mercuric transport protein periplasmic component	-
K6K06_RS27410 (TUM12138_52930)	60102..60791	-	690	Protein_67	AAA family ATPase	-
K6K06_RS27415 (TUM12138_52940)	60865..61281	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6K06_RS27420 (TUM12138_52950)	61278..61508	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6K06_RS27425	61465..61926	+	462	WP_072202616.1	hypothetical protein	-
K6K06_RS27430 (TUM12138_52960)	62070..62420	+	351	WP_000493378.1	hypothetical protein	-
K6K06_RS27435 (TUM12138_52970)	62471..63214	+	744	WP_000129823.1	hypothetical protein	-
K6K06_RS27440 (TUM12138_52980)	63211..63987	+	777	WP_000015958.1	site-specific integrase	-
K6K06_RS28040 (TUM12138_52990)	64045..64224	-	180	Protein_74	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia	-	1..123780	123780
	-	inside	IScluster/Tn	aac(6')-Ib	-	59350..69826	10476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T39681 WP_001044770.1 NZ_AP024790:c61281-60865 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T39681 NZ_AP024790:c61281-60865 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT39681 WP_001261282.1 NZ_AP024790:c61508-61278 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT39681 NZ_AP024790:c61508-61278 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3