Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60865..61508 Replicon plasmid IncN-R
Accession NZ_AP024790
Organism Klebsiella pneumoniae strain TUM12138

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6K06_RS27415 Protein ID WP_001044770.1
Coordinates 60865..61281 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6K06_RS27420 Protein ID WP_001261282.1
Coordinates 61278..61508 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6K06_RS27380 (TUM12138_52870) 56407..56643 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
K6K06_RS27385 (TUM12138_52880) 56640..57002 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6K06_RS27390 (TUM12138_52890) 57020..58714 - 1695 WP_000105636.1 mercury(II) reductase -
K6K06_RS27395 (TUM12138_52900) 58766..59188 - 423 WP_001340589.1 organomercurial transporter MerC -
K6K06_RS27400 59224..59334 - 111 Protein_65 mercuric transport protein periplasmic component -
K6K06_RS27410 (TUM12138_52930) 60102..60791 - 690 Protein_67 AAA family ATPase -
K6K06_RS27415 (TUM12138_52940) 60865..61281 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6K06_RS27420 (TUM12138_52950) 61278..61508 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6K06_RS27425 61465..61926 + 462 WP_072202616.1 hypothetical protein -
K6K06_RS27430 (TUM12138_52960) 62070..62420 + 351 WP_000493378.1 hypothetical protein -
K6K06_RS27435 (TUM12138_52970) 62471..63214 + 744 WP_000129823.1 hypothetical protein -
K6K06_RS27440 (TUM12138_52980) 63211..63987 + 777 WP_000015958.1 site-specific integrase -
K6K06_RS28040 (TUM12138_52990) 64045..64224 - 180 Protein_74 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..123780 123780
- inside IScluster/Tn aac(6')-Ib - 59350..69826 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39681 WP_001044770.1 NZ_AP024790:c61281-60865 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39681 NZ_AP024790:c61281-60865 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39681 WP_001261282.1 NZ_AP024790:c61508-61278 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39681 NZ_AP024790:c61508-61278 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References