Detailed information of TA system TA039678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 36880..37619
• Replicon: plasmid pMTY12138
• Accession: NZ_AP024789
• Organism: Klebsiella pneumoniae strain TUM12138

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: K6K06_RS26200
• Protein ID: WP_021312536.1
• Coordinates: 37134..37619 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: K6K06_RS26195
• Protein ID: WP_049095044.1
• Coordinates: 36880..37146 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6K06_RS26170 (TUM12138_50320)	32775..33218	+	444	WP_004026357.1	DUF2384 domain-containing protein	-
K6K06_RS26175 (TUM12138_50330)	33215..33685	+	471	WP_004883378.1	RES family NAD+ phosphorylase	-
K6K06_RS26180 (TUM12138_50340)	33794..34054	-	261	WP_004026352.1	hypothetical protein	-
K6K06_RS26185	34749..36118	+	1370	WP_087661561.1	IS3 family transposase	-
K6K06_RS26190 (TUM12138_50370)	36319..36747	+	429	WP_004901287.1	GFA family protein	-
K6K06_RS26195 (TUM12138_50380)	36880..37146	+	267	WP_049095044.1	DUF1778 domain-containing protein	Antitoxin
K6K06_RS26200	37134..37619	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
K6K06_RS26205	37957..38991	-	1035	Protein_44	IS481 family transposase	-
K6K06_RS26210 (TUM12138_50400)	39063..39221	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
K6K06_RS26215 (TUM12138_50410)	39827..40801	+	975	WP_181849761.1	Hok/Gef family protein	-
K6K06_RS28135	40785..40907	+	123	WP_255308559.1	hypothetical protein	-
K6K06_RS26220 (TUM12138_50420)	40999..41376	-	378	WP_004883386.1	hypothetical protein	-
K6K06_RS26225 (TUM12138_50430)	41691..41903	+	213	WP_046654973.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..216384	216384
	-	inside	IScluster/Tn	-	-	30898..38991	8093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T39678 WP_021312536.1 NZ_AP024789:37134-37619 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T39678 NZ_AP024789:37134-37619 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10163.63 Da
• Isoelectric Point: 4.5256

>AT39678 WP_049095044.1 NZ_AP024789:36880-37146 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDELAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT39678 NZ_AP024789:36880-37146 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCTCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA