Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60459..61102 Replicon plasmid IncN-R
Accession NZ_AP024786
Organism Klebsiella pneumoniae strain TUM12137

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6K11_RS27410 Protein ID WP_001044770.1
Coordinates 60459..60875 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6K11_RS27415 Protein ID WP_001261282.1
Coordinates 60872..61102 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6K11_RS27375 (TUM12137_52850) 56001..56237 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
K6K11_RS27380 (TUM12137_52860) 56234..56596 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6K11_RS27385 (TUM12137_52870) 56614..58308 - 1695 WP_000105636.1 mercury(II) reductase -
K6K11_RS27390 (TUM12137_52880) 58360..58782 - 423 WP_001340589.1 organomercurial transporter MerC -
K6K11_RS27395 58818..58928 - 111 Protein_65 mercuric transport protein periplasmic component -
K6K11_RS27405 (TUM12137_52910) 59696..60385 - 690 Protein_67 AAA family ATPase -
K6K11_RS27410 (TUM12137_52920) 60459..60875 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6K11_RS27415 (TUM12137_52930) 60872..61102 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6K11_RS27420 61059..61520 + 462 WP_072202616.1 hypothetical protein -
K6K11_RS27425 (TUM12137_52940) 62078..63397 + 1320 WP_004152397.1 IS1182-like element ISKpn6 family transposase -
K6K11_RS27430 (TUM12137_52950) 63647..64528 - 882 WP_004199234.1 carbapenem-hydrolyzing class A beta-lactamase KPC-2 -
K6K11_RS27435 (TUM12137_52960) 64816..65595 - 780 WP_004152394.1 IS21-like element ISKpn7 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..128491 128491
- inside IScluster/Tn blaKPC-2 / aac(6')-Ib - 58944..75877 16933


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39658 WP_001044770.1 NZ_AP024786:c60875-60459 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39658 NZ_AP024786:c60875-60459 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39658 WP_001261282.1 NZ_AP024786:c61102-60872 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39658 NZ_AP024786:c61102-60872 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References