Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4076977..4077596 Replicon chromosome
Accession NZ_AP024781
Organism Klebsiella pneumoniae strain TUM12136

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J90_RS20265 Protein ID WP_002892050.1
Coordinates 4077378..4077596 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J90_RS20260 Protein ID WP_002892066.1
Coordinates 4076977..4077351 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J90_RS20250 (TUM12136_39060) 4072129..4073322 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6J90_RS20255 (TUM12136_39070) 4073345..4076491 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J90_RS20260 (TUM12136_39080) 4076977..4077351 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J90_RS20265 (TUM12136_39090) 4077378..4077596 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J90_RS20270 (TUM12136_39100) 4077755..4078321 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6J90_RS20275 4078293..4078433 - 141 WP_004147370.1 hypothetical protein -
K6J90_RS20280 (TUM12136_39110) 4078454..4078924 + 471 WP_002892026.1 YlaC family protein -
K6J90_RS20285 (TUM12136_39120) 4078899..4080350 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J90_RS20290 (TUM12136_39130) 4080451..4081149 + 699 WP_002892021.1 GNAT family protein -
K6J90_RS20295 (TUM12136_39140) 4081146..4081286 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J90_RS20300 (TUM12136_39150) 4081286..4081549 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39630 WP_002892050.1 NZ_AP024781:4077378-4077596 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39630 NZ_AP024781:4077378-4077596 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39630 WP_002892066.1 NZ_AP024781:4076977-4077351 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39630 NZ_AP024781:4076977-4077351 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References