Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4012235..4012854 Replicon chromosome
Accession NZ_AP024775
Organism Klebsiella pneumoniae strain TUM12134

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J97_RS19860 Protein ID WP_002892050.1
Coordinates 4012636..4012854 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J97_RS19855 Protein ID WP_002892066.1
Coordinates 4012235..4012609 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J97_RS19845 (TUM12134_38300) 4007387..4008580 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6J97_RS19850 (TUM12134_38310) 4008603..4011749 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J97_RS19855 (TUM12134_38320) 4012235..4012609 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J97_RS19860 (TUM12134_38330) 4012636..4012854 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J97_RS19865 (TUM12134_38340) 4013013..4013579 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6J97_RS19870 4013551..4013691 - 141 WP_004147370.1 hypothetical protein -
K6J97_RS19875 (TUM12134_38350) 4013712..4014182 + 471 WP_002892026.1 YlaC family protein -
K6J97_RS19880 (TUM12134_38360) 4014157..4015608 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J97_RS19885 (TUM12134_38370) 4015709..4016407 + 699 WP_221270784.1 GNAT family protein -
K6J97_RS19890 (TUM12134_38380) 4016404..4016544 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J97_RS19895 (TUM12134_38390) 4016544..4016807 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39592 WP_002892050.1 NZ_AP024775:4012636-4012854 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39592 NZ_AP024775:4012636-4012854 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39592 WP_002892066.1 NZ_AP024775:4012235-4012609 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39592 NZ_AP024775:4012235-4012609 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References