Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5207789..5208414 Replicon chromosome
Accession NZ_AP024772
Organism Klebsiella pneumoniae strain TUM12133

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6J98_RS25610 Protein ID WP_002882817.1
Coordinates 5207789..5208172 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6J98_RS25615 Protein ID WP_004150355.1
Coordinates 5208172..5208414 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J98_RS25595 (TUM12133_49320) 5205155..5206057 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6J98_RS25600 (TUM12133_49330) 5206054..5206689 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6J98_RS25605 (TUM12133_49340) 5206686..5207615 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6J98_RS25610 (TUM12133_49350) 5207789..5208172 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J98_RS25615 (TUM12133_49360) 5208172..5208414 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6J98_RS25620 (TUM12133_49370) 5208619..5209536 + 918 WP_002882812.1 alpha/beta hydrolase -
K6J98_RS25625 (TUM12133_49380) 5209550..5210491 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6J98_RS25630 (TUM12133_49390) 5210536..5210973 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6J98_RS25635 (TUM12133_49400) 5210970..5211830 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6J98_RS25640 (TUM12133_49410) 5211824..5212423 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T39577 WP_002882817.1 NZ_AP024772:c5208172-5207789 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T39577 NZ_AP024772:c5208172-5207789 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT39577 WP_004150355.1 NZ_AP024772:c5208414-5208172 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT39577 NZ_AP024772:c5208414-5208172 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References