Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60906..61549 Replicon plasmid IncN-R
Accession NZ_AP024770
Organism Klebsiella pneumoniae strain TUM12132

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6J84_RS26310 Protein ID WP_001044770.1
Coordinates 60906..61322 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6J84_RS26315 Protein ID WP_001261282.1
Coordinates 61319..61549 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J84_RS26275 (TUM12132_50430) 56448..56684 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
K6J84_RS26280 (TUM12132_50440) 56681..57043 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6J84_RS26285 (TUM12132_50450) 57061..58755 - 1695 WP_000105636.1 mercury(II) reductase -
K6J84_RS26290 (TUM12132_50460) 58807..59229 - 423 WP_001340589.1 organomercurial transporter MerC -
K6J84_RS26295 59265..59375 - 111 Protein_66 mercuric transport protein periplasmic component -
K6J84_RS26305 (TUM12132_50490) 60143..60832 - 690 Protein_68 AAA family ATPase -
K6J84_RS26310 (TUM12132_50500) 60906..61322 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J84_RS26315 (TUM12132_50510) 61319..61549 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6J84_RS26320 61506..61967 + 462 WP_072202616.1 hypothetical protein -
K6J84_RS26325 (TUM12132_50520) 62111..62461 + 351 WP_000493378.1 hypothetical protein -
K6J84_RS26330 (TUM12132_50530) 62512..63255 + 744 WP_000129823.1 hypothetical protein -
K6J84_RS26335 (TUM12132_50540) 63252..64028 + 777 WP_000015958.1 site-specific integrase -
K6J84_RS26920 (TUM12132_50550) 64086..64265 - 180 Protein_75 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..123805 123805
- inside IScluster/Tn aac(6')-Ib - 59391..69867 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39559 WP_001044770.1 NZ_AP024770:c61322-60906 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39559 NZ_AP024770:c61322-60906 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39559 WP_001261282.1 NZ_AP024770:c61549-61319 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39559 NZ_AP024770:c61549-61319 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References