Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4012826..4013445 Replicon chromosome
Accession NZ_AP024766
Organism Klebsiella pneumoniae strain TUM12131

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J85_RS19865 Protein ID WP_002892050.1
Coordinates 4013227..4013445 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J85_RS19860 Protein ID WP_002892066.1
Coordinates 4012826..4013200 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J85_RS19850 (TUM12131_38160) 4007978..4009171 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6J85_RS19855 (TUM12131_38170) 4009194..4012340 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J85_RS19860 (TUM12131_38180) 4012826..4013200 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J85_RS19865 (TUM12131_38190) 4013227..4013445 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J85_RS19870 (TUM12131_38200) 4013604..4014170 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6J85_RS19875 4014142..4014282 - 141 WP_004147370.1 hypothetical protein -
K6J85_RS19880 (TUM12131_38210) 4014303..4014773 + 471 WP_002892026.1 YlaC family protein -
K6J85_RS19885 (TUM12131_38220) 4014748..4016199 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J85_RS19890 (TUM12131_38230) 4016300..4016998 + 699 WP_002892021.1 GNAT family protein -
K6J85_RS19895 (TUM12131_38240) 4016995..4017135 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J85_RS19900 (TUM12131_38250) 4017135..4017398 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39535 WP_002892050.1 NZ_AP024766:4013227-4013445 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39535 NZ_AP024766:4013227-4013445 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39535 WP_002892066.1 NZ_AP024766:4012826-4013200 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39535 NZ_AP024766:4012826-4013200 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References