Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60843..61486 Replicon plasmid IncN-R
Accession NZ_AP024761
Organism Klebsiella pneumoniae strain TUM12129

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6J88_RS26740 Protein ID WP_001044770.1
Coordinates 60843..61259 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6J88_RS26745 Protein ID WP_001261282.1
Coordinates 61256..61486 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J88_RS26705 (TUM12129_51530) 56385..56621 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
K6J88_RS26710 (TUM12129_51540) 56618..56980 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6J88_RS26715 (TUM12129_51550) 56998..58692 - 1695 WP_000105636.1 mercury(II) reductase -
K6J88_RS26720 (TUM12129_51560) 58744..59166 - 423 WP_001340589.1 organomercurial transporter MerC -
K6J88_RS26725 59202..59312 - 111 Protein_65 mercuric transport protein periplasmic component -
K6J88_RS26735 (TUM12129_51590) 60080..60769 - 690 Protein_67 AAA family ATPase -
K6J88_RS26740 (TUM12129_51600) 60843..61259 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J88_RS26745 (TUM12129_51610) 61256..61486 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6J88_RS26750 61443..61904 + 462 WP_072202616.1 hypothetical protein -
K6J88_RS26755 (TUM12129_51620) 62048..62398 + 351 WP_000493378.1 hypothetical protein -
K6J88_RS26760 (TUM12129_51630) 62449..63192 + 744 WP_000129823.1 hypothetical protein -
K6J88_RS26765 (TUM12129_51640) 63189..63965 + 777 WP_000015958.1 site-specific integrase -
K6J88_RS27415 (TUM12129_51650) 64023..64202 - 180 Protein_74 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaTEM-54 / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..125298 125298
- inside IScluster/Tn aac(6')-Ib - 59328..69804 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39502 WP_001044770.1 NZ_AP024761:c61259-60843 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39502 NZ_AP024761:c61259-60843 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39502 WP_001261282.1 NZ_AP024761:c61486-61256 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39502 NZ_AP024761:c61486-61256 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References