Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5275537..5276162 Replicon chromosome
Accession NZ_AP024760
Organism Klebsiella pneumoniae strain TUM12129

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6J88_RS26035 Protein ID WP_002882817.1
Coordinates 5275537..5275920 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6J88_RS26040 Protein ID WP_004150355.1
Coordinates 5275920..5276162 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J88_RS26020 (TUM12129_50180) 5272903..5273805 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6J88_RS26025 (TUM12129_50190) 5273802..5274437 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6J88_RS26030 (TUM12129_50200) 5274434..5275363 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6J88_RS26035 (TUM12129_50210) 5275537..5275920 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J88_RS26040 (TUM12129_50220) 5275920..5276162 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6J88_RS26045 (TUM12129_50230) 5276367..5277284 + 918 WP_002882812.1 alpha/beta hydrolase -
K6J88_RS26050 (TUM12129_50240) 5277298..5278239 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6J88_RS26055 (TUM12129_50250) 5278284..5278721 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6J88_RS26060 (TUM12129_50260) 5278718..5279578 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6J88_RS26065 (TUM12129_50270) 5279572..5280171 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T39501 WP_002882817.1 NZ_AP024760:c5275920-5275537 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T39501 NZ_AP024760:c5275920-5275537 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT39501 WP_004150355.1 NZ_AP024760:c5276162-5275920 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT39501 NZ_AP024760:c5276162-5275920 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References