Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4206592..4207211 Replicon chromosome
Accession NZ_AP024756
Organism Klebsiella pneumoniae strain TUM12128

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J96_RS20595 Protein ID WP_002892050.1
Coordinates 4206592..4206810 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J96_RS20600 Protein ID WP_002892066.1
Coordinates 4206837..4207211 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J96_RS20560 (TUM12128_39370) 4202639..4202902 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
K6J96_RS20565 (TUM12128_39380) 4202902..4203042 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J96_RS20570 (TUM12128_39390) 4203039..4203737 - 699 WP_002892021.1 GNAT family protein -
K6J96_RS20575 (TUM12128_39400) 4203838..4205289 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J96_RS20580 (TUM12128_39410) 4205264..4205734 - 471 WP_002892026.1 YlaC family protein -
K6J96_RS20585 4205755..4205895 + 141 WP_004147370.1 hypothetical protein -
K6J96_RS20590 (TUM12128_39420) 4205867..4206433 - 567 WP_002892030.1 maltose O-acetyltransferase -
K6J96_RS20595 (TUM12128_39430) 4206592..4206810 - 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J96_RS20600 (TUM12128_39440) 4206837..4207211 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J96_RS20605 (TUM12128_39450) 4207697..4210843 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J96_RS20610 (TUM12128_39460) 4210866..4212059 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39482 WP_002892050.1 NZ_AP024756:c4206810-4206592 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39482 NZ_AP024756:c4206810-4206592 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39482 WP_002892066.1 NZ_AP024756:c4207211-4206837 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39482 NZ_AP024756:c4207211-4206837 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References