Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3008391..3009016 Replicon chromosome
Accession NZ_AP024756
Organism Klebsiella pneumoniae strain TUM12128

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6J96_RS14845 Protein ID WP_002882817.1
Coordinates 3008633..3009016 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6J96_RS14840 Protein ID WP_004150355.1
Coordinates 3008391..3008633 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J96_RS14815 (TUM12128_28400) 3004382..3004981 + 600 WP_004151865.1 glucose-1-phosphatase -
K6J96_RS14820 (TUM12128_28410) 3004975..3005835 + 861 WP_002882807.1 virulence factor BrkB family protein -
K6J96_RS14825 (TUM12128_28420) 3005832..3006269 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6J96_RS14830 (TUM12128_28430) 3006314..3007255 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6J96_RS14835 (TUM12128_28440) 3007269..3008186 - 918 WP_002882812.1 alpha/beta hydrolase -
K6J96_RS14840 (TUM12128_28450) 3008391..3008633 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6J96_RS14845 (TUM12128_28460) 3008633..3009016 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J96_RS14850 (TUM12128_28470) 3009190..3010119 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6J96_RS14855 (TUM12128_28480) 3010116..3010751 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6J96_RS14860 (TUM12128_28490) 3010748..3011650 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T39478 WP_002882817.1 NZ_AP024756:3008633-3009016 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T39478 NZ_AP024756:3008633-3009016 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT39478 WP_004150355.1 NZ_AP024756:3008391-3008633 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT39478 NZ_AP024756:3008391-3008633 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References