Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60874..61517 Replicon plasmid IncN-R
Accession NZ_AP024754
Organism Klebsiella pneumoniae strain TUM12127

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6J87_RS26730 Protein ID WP_001044770.1
Coordinates 60874..61290 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6J87_RS26735 Protein ID WP_001261282.1
Coordinates 61287..61517 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J87_RS26695 (TUM12127_51370) 56416..56652 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
K6J87_RS26700 (TUM12127_51380) 56649..57011 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
K6J87_RS26705 (TUM12127_51390) 57029..58723 - 1695 WP_000105636.1 mercury(II) reductase -
K6J87_RS26710 (TUM12127_51400) 58775..59197 - 423 WP_001340589.1 organomercurial transporter MerC -
K6J87_RS26715 59233..59343 - 111 Protein_65 mercuric transport protein periplasmic component -
K6J87_RS26725 (TUM12127_51430) 60111..60800 - 690 Protein_67 AAA family ATPase -
K6J87_RS26730 (TUM12127_51440) 60874..61290 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6J87_RS26735 (TUM12127_51450) 61287..61517 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6J87_RS26740 61474..61935 + 462 WP_072202616.1 hypothetical protein -
K6J87_RS26745 (TUM12127_51460) 62079..62429 + 351 WP_000493378.1 hypothetical protein -
K6J87_RS26750 (TUM12127_51470) 62480..63223 + 744 WP_000129823.1 hypothetical protein -
K6J87_RS26755 (TUM12127_51480) 63220..63996 + 777 WP_000015958.1 site-specific integrase -
K6J87_RS27370 (TUM12127_51490) 64054..64233 - 180 Protein_74 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaOXA-9 / blaTEM-1A / aac(6')-Ib / aadA2 / cmlA1 / ant(3'')-Ia / aac(3)-IVa / aph(4)-Ia - 1..123514 123514
- inside IScluster/Tn aac(6')-Ib - 59359..69835 10476


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T39464 WP_001044770.1 NZ_AP024754:c61290-60874 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T39464 NZ_AP024754:c61290-60874 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT39464 WP_001261282.1 NZ_AP024754:c61517-61287 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT39464 NZ_AP024754:c61517-61287 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References