Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4074731..4075350 Replicon chromosome
Accession NZ_AP024753
Organism Klebsiella pneumoniae strain TUM12127

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J87_RS20275 Protein ID WP_002892050.1
Coordinates 4075132..4075350 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J87_RS20270 Protein ID WP_002892066.1
Coordinates 4074731..4075105 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J87_RS20260 (TUM12127_39080) 4069883..4071076 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6J87_RS20265 (TUM12127_39090) 4071099..4074245 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J87_RS20270 (TUM12127_39100) 4074731..4075105 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J87_RS20275 (TUM12127_39110) 4075132..4075350 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J87_RS20280 (TUM12127_39120) 4075509..4076075 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6J87_RS20285 4076047..4076187 - 141 WP_004147370.1 hypothetical protein -
K6J87_RS20290 (TUM12127_39130) 4076208..4076678 + 471 WP_002892026.1 YlaC family protein -
K6J87_RS20295 (TUM12127_39140) 4076653..4078104 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J87_RS20300 (TUM12127_39150) 4078205..4078903 + 699 WP_002892021.1 GNAT family protein -
K6J87_RS20305 (TUM12127_39160) 4078900..4079040 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J87_RS20310 (TUM12127_39170) 4079040..4079303 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39459 WP_002892050.1 NZ_AP024753:4075132-4075350 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39459 NZ_AP024753:4075132-4075350 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39459 WP_002892066.1 NZ_AP024753:4074731-4075105 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39459 NZ_AP024753:4074731-4075105 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References