Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4014767..4015386 Replicon chromosome
Accession NZ_AP024750
Organism Klebsiella pneumoniae strain TUM12126

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6J82_RS19885 Protein ID WP_002892050.1
Coordinates 4015168..4015386 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6J82_RS19880 Protein ID WP_002892066.1
Coordinates 4014767..4015141 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J82_RS19870 (TUM12126_38280) 4009919..4011112 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6J82_RS19875 (TUM12126_38290) 4011135..4014281 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6J82_RS19880 (TUM12126_38300) 4014767..4015141 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6J82_RS19885 (TUM12126_38310) 4015168..4015386 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6J82_RS19890 (TUM12126_38320) 4015545..4016111 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6J82_RS19895 4016083..4016223 - 141 WP_004147370.1 hypothetical protein -
K6J82_RS19900 (TUM12126_38330) 4016244..4016714 + 471 WP_002892026.1 YlaC family protein -
K6J82_RS19905 (TUM12126_38340) 4016689..4018140 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6J82_RS19910 (TUM12126_38350) 4018241..4018939 + 699 WP_002892021.1 GNAT family protein -
K6J82_RS19915 (TUM12126_38360) 4018936..4019076 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6J82_RS19920 (TUM12126_38370) 4019076..4019339 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T39440 WP_002892050.1 NZ_AP024750:4015168-4015386 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T39440 NZ_AP024750:4015168-4015386 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT39440 WP_002892066.1 NZ_AP024750:4014767-4015141 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT39440 NZ_AP024750:4014767-4015141 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References