Detailed information of TA system TA039413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2188600..2189129
• Replicon: chromosome
• Accession: NZ_AP024742
• Organism: Staphylococcus aureus strain 59736

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX51_RS10945
• Protein ID: WP_000621175.1
• Coordinates: 2188600..2188962 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX51_RS10950
• Protein ID: WP_000948331.1
• Coordinates: 2188959..2189129 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX51_RS10925 (2185578)	2185578..2186348	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX51_RS10930 (2186323)	2186323..2186802	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX51_RS10935 (2186804)	2186804..2187130	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX51_RS10940 (2187249)	2187249..2188250	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX51_RS10945 (2188600)	2188600..2188962	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX51_RS10950 (2188959)	2188959..2189129	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX51_RS10955 (2189214)	2189214..2190362	-	1149	WP_001281154.1	alanine racemase	-
OCX51_RS10960 (2190428)	2190428..2190787	-	360	WP_000581197.1	holo-ACP synthase	-
OCX51_RS10965 (2190791)	2190791..2191282	-	492	WP_001205912.1	PH domain-containing protein	-
OCX51_RS10970 (2191269)	2191269..2192852	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX51_RS10975 (2192845)	2192845..2193324	-	480	WP_001287078.1	hypothetical protein	-
OCX51_RS10980 (2193533)	2193533..2194093	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39413 WP_000621175.1 NZ_AP024742:c2188962-2188600 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39413 NZ_AP024742:c2188962-2188600 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39413 WP_000948331.1 NZ_AP024742:c2189129-2188959 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39413 NZ_AP024742:c2189129-2188959 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1