Detailed information of TA system TA039393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2185394..2185923
• Replicon: chromosome
• Accession: NZ_AP024738
• Organism: Staphylococcus aureus strain 59731

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX20_RS10930
• Protein ID: WP_000621175.1
• Coordinates: 2185394..2185756 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX20_RS10935
• Protein ID: WP_000948331.1
• Coordinates: 2185753..2185923 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX20_RS10910 (2182372)	2182372..2183142	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX20_RS10915 (2183117)	2183117..2183596	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX20_RS10920 (2183598)	2183598..2183924	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX20_RS10925 (2184043)	2184043..2185044	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX20_RS10930 (2185394)	2185394..2185756	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX20_RS10935 (2185753)	2185753..2185923	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX20_RS10940 (2186008)	2186008..2187156	-	1149	WP_001281154.1	alanine racemase	-
OCX20_RS10945 (2187222)	2187222..2187581	-	360	WP_000581197.1	holo-ACP synthase	-
OCX20_RS10950 (2187585)	2187585..2188076	-	492	WP_001205912.1	PH domain-containing protein	-
OCX20_RS10955 (2188063)	2188063..2189646	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX20_RS10960 (2189639)	2189639..2190118	-	480	WP_001287078.1	hypothetical protein	-
OCX20_RS10965 (2190327)	2190327..2190887	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39393 WP_000621175.1 NZ_AP024738:c2185756-2185394 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39393 NZ_AP024738:c2185756-2185394 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39393 WP_000948331.1 NZ_AP024738:c2185923-2185753 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39393 NZ_AP024738:c2185923-2185753 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1