Detailed information of TA system TA039373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2173090..2173619
• Replicon: chromosome
• Accession: NZ_AP024736
• Organism: Staphylococcus aureus strain 59709

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX48_RS10885
• Protein ID: WP_000621175.1
• Coordinates: 2173090..2173452 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX48_RS10890
• Protein ID: WP_000948331.1
• Coordinates: 2173449..2173619 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX48_RS10865 (2170068)	2170068..2170838	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX48_RS10870 (2170813)	2170813..2171292	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX48_RS10875 (2171294)	2171294..2171620	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX48_RS10880 (2171739)	2171739..2172740	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX48_RS10885 (2173090)	2173090..2173452	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX48_RS10890 (2173449)	2173449..2173619	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX48_RS10895 (2173704)	2173704..2174852	-	1149	WP_001281154.1	alanine racemase	-
OCX48_RS10900 (2174918)	2174918..2175277	-	360	WP_000581197.1	holo-ACP synthase	-
OCX48_RS10905 (2175281)	2175281..2175772	-	492	WP_001205912.1	PH domain-containing protein	-
OCX48_RS10910 (2175759)	2175759..2177342	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX48_RS10915 (2177335)	2177335..2177814	-	480	WP_001287078.1	hypothetical protein	-
OCX48_RS10920 (2178023)	2178023..2178583	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39373 WP_000621175.1 NZ_AP024736:c2173452-2173090 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39373 NZ_AP024736:c2173452-2173090 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39373 WP_000948331.1 NZ_AP024736:c2173619-2173449 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39373 NZ_AP024736:c2173619-2173449 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1