Detailed information of TA system TA039353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195220..2195749
• Replicon: chromosome
• Accession: NZ_AP024734
• Organism: Staphylococcus aureus strain 59691

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX19_RS11035
• Protein ID: WP_000621175.1
• Coordinates: 2195220..2195582 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX19_RS11040
• Protein ID: WP_000948331.1
• Coordinates: 2195579..2195749 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX19_RS11015 (2192199)	2192199..2192969	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX19_RS11020 (2192944)	2192944..2193423	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX19_RS11025 (2193425)	2193425..2193751	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX19_RS11030 (2193870)	2193870..2194871	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX19_RS11035 (2195220)	2195220..2195582	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX19_RS11040 (2195579)	2195579..2195749	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX19_RS11045 (2195834)	2195834..2196982	-	1149	WP_001281154.1	alanine racemase	-
OCX19_RS11050 (2197048)	2197048..2197407	-	360	WP_000581197.1	holo-ACP synthase	-
OCX19_RS11055 (2197411)	2197411..2197902	-	492	WP_001205912.1	PH domain-containing protein	-
OCX19_RS11060 (2197889)	2197889..2199472	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX19_RS11065 (2199465)	2199465..2199944	-	480	WP_001287078.1	hypothetical protein	-
OCX19_RS11070 (2200153)	2200153..2200713	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39353 WP_000621175.1 NZ_AP024734:c2195582-2195220 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39353 NZ_AP024734:c2195582-2195220 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39353 WP_000948331.1 NZ_AP024734:c2195749-2195579 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39353 NZ_AP024734:c2195749-2195579 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1