Detailed information of TA system TA039333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2136471..2137000
• Replicon: chromosome
• Accession: NZ_AP024732
• Organism: Staphylococcus aureus strain 59621

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX17_RS10625
• Protein ID: WP_000621175.1
• Coordinates: 2136471..2136833 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX17_RS10630
• Protein ID: WP_000948331.1
• Coordinates: 2136830..2137000 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX17_RS10605 (2133449)	2133449..2134219	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX17_RS10610 (2134194)	2134194..2134673	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX17_RS10615 (2134675)	2134675..2135001	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX17_RS10620 (2135120)	2135120..2136121	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX17_RS10625 (2136471)	2136471..2136833	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX17_RS10630 (2136830)	2136830..2137000	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX17_RS10635 (2137085)	2137085..2138233	-	1149	WP_001281154.1	alanine racemase	-
OCX17_RS10640 (2138299)	2138299..2138658	-	360	WP_000581197.1	holo-ACP synthase	-
OCX17_RS10645 (2138662)	2138662..2139153	-	492	WP_001205912.1	PH domain-containing protein	-
OCX17_RS10650 (2139140)	2139140..2140723	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX17_RS10655 (2140716)	2140716..2141195	-	480	WP_001287078.1	hypothetical protein	-
OCX17_RS10660 (2141404)	2141404..2141964	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39333 WP_000621175.1 NZ_AP024732:c2136833-2136471 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39333 NZ_AP024732:c2136833-2136471 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39333 WP_000948331.1 NZ_AP024732:c2137000-2136830 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39333 NZ_AP024732:c2137000-2136830 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1