Detailed information of TA system TA039317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2178950..2179479
• Replicon: chromosome
• Accession: NZ_AP024730
• Organism: Staphylococcus aureus strain 59574

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX16_RS10905
• Protein ID: WP_000621175.1
• Coordinates: 2178950..2179312 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX16_RS10910
• Protein ID: WP_000948331.1
• Coordinates: 2179309..2179479 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX16_RS10885 (2175928)	2175928..2176698	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX16_RS10890 (2176673)	2176673..2177152	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX16_RS10895 (2177154)	2177154..2177480	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX16_RS10900 (2177599)	2177599..2178600	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX16_RS10905 (2178950)	2178950..2179312	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX16_RS10910 (2179309)	2179309..2179479	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX16_RS10915 (2179564)	2179564..2180712	-	1149	WP_001281154.1	alanine racemase	-
OCX16_RS10920 (2180778)	2180778..2181137	-	360	WP_000581197.1	holo-ACP synthase	-
OCX16_RS10925 (2181141)	2181141..2181632	-	492	WP_001205912.1	PH domain-containing protein	-
OCX16_RS10930 (2181619)	2181619..2183202	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX16_RS10935 (2183195)	2183195..2183674	-	480	WP_001287078.1	hypothetical protein	-
OCX16_RS10940 (2183883)	2183883..2184443	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39317 WP_000621175.1 NZ_AP024730:c2179312-2178950 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39317 NZ_AP024730:c2179312-2178950 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39317 WP_000948331.1 NZ_AP024730:c2179479-2179309 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39317 NZ_AP024730:c2179479-2179309 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1