Detailed information of TA system TA039297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2140906..2141435
• Replicon: chromosome
• Accession: NZ_AP024728
• Organism: Staphylococcus aureus strain 59553

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX18_RS10615
• Protein ID: WP_000621175.1
• Coordinates: 2140906..2141268 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX18_RS10620
• Protein ID: WP_000948331.1
• Coordinates: 2141265..2141435 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX18_RS10595 (2137884)	2137884..2138654	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX18_RS10600 (2138629)	2138629..2139108	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX18_RS10605 (2139110)	2139110..2139436	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX18_RS10610 (2139555)	2139555..2140556	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX18_RS10615 (2140906)	2140906..2141268	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX18_RS10620 (2141265)	2141265..2141435	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX18_RS10625 (2141520)	2141520..2142668	-	1149	WP_001281154.1	alanine racemase	-
OCX18_RS10630 (2142734)	2142734..2143093	-	360	WP_000581197.1	holo-ACP synthase	-
OCX18_RS10635 (2143097)	2143097..2143588	-	492	WP_001205912.1	PH domain-containing protein	-
OCX18_RS10640 (2143575)	2143575..2145158	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX18_RS10645 (2145151)	2145151..2145630	-	480	WP_001287078.1	hypothetical protein	-
OCX18_RS10650 (2145839)	2145839..2146399	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39297 WP_000621175.1 NZ_AP024728:c2141268-2140906 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39297 NZ_AP024728:c2141268-2140906 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39297 WP_000948331.1 NZ_AP024728:c2141435-2141265 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39297 NZ_AP024728:c2141435-2141265 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1