Detailed information of TA system TA039282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2165670..2166199
• Replicon: chromosome
• Accession: NZ_AP024726
• Organism: Staphylococcus aureus strain 59482

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX11_RS10855
• Protein ID: WP_000621175.1
• Coordinates: 2165670..2166032 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX11_RS10860
• Protein ID: WP_000948331.1
• Coordinates: 2166029..2166199 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX11_RS10835 (2162648)	2162648..2163418	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX11_RS10840 (2163393)	2163393..2163872	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX11_RS10845 (2163874)	2163874..2164200	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX11_RS10850 (2164319)	2164319..2165320	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX11_RS10855 (2165670)	2165670..2166032	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX11_RS10860 (2166029)	2166029..2166199	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX11_RS10865 (2166284)	2166284..2167432	-	1149	WP_001281154.1	alanine racemase	-
OCX11_RS10870 (2167498)	2167498..2167857	-	360	WP_000581197.1	holo-ACP synthase	-
OCX11_RS10875 (2167861)	2167861..2168352	-	492	WP_001205912.1	PH domain-containing protein	-
OCX11_RS10880 (2168339)	2168339..2169922	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX11_RS10885 (2169915)	2169915..2170394	-	480	WP_001287078.1	hypothetical protein	-
OCX11_RS10890 (2170603)	2170603..2171163	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39282 WP_000621175.1 NZ_AP024726:c2166032-2165670 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39282 NZ_AP024726:c2166032-2165670 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39282 WP_000948331.1 NZ_AP024726:c2166199-2166029 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39282 NZ_AP024726:c2166199-2166029 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1