Detailed information of TA system TA039262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2183032..2183561
• Replicon: chromosome
• Accession: NZ_AP024723
• Organism: Staphylococcus aureus strain 59458

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCX22_RS10920
• Protein ID: WP_000621175.1
• Coordinates: 2183032..2183394 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: OCX22_RS10925
• Protein ID: WP_000948331.1
• Coordinates: 2183391..2183561 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCX22_RS10900 (2180011)	2180011..2180781	-	771	WP_117201032.1	RNA polymerase sigma factor SigB	-
OCX22_RS10905 (2180756)	2180756..2181235	-	480	WP_117201034.1	anti-sigma B factor RsbW	-
OCX22_RS10910 (2181237)	2181237..2181563	-	327	WP_001052491.1	anti-sigma factor antagonist	-
OCX22_RS10915 (2181682)	2181682..2182683	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
OCX22_RS10920 (2183032)	2183032..2183394	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCX22_RS10925 (2183391)	2183391..2183561	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
OCX22_RS10930 (2183646)	2183646..2184794	-	1149	WP_001281154.1	alanine racemase	-
OCX22_RS10935 (2184860)	2184860..2185219	-	360	WP_000581197.1	holo-ACP synthase	-
OCX22_RS10940 (2185223)	2185223..2185714	-	492	WP_001205912.1	PH domain-containing protein	-
OCX22_RS10945 (2185701)	2185701..2187284	-	1584	WP_001294626.1	PH domain-containing protein	-
OCX22_RS10950 (2187277)	2187277..2187756	-	480	WP_001287078.1	hypothetical protein	-
OCX22_RS10955 (2187965)	2187965..2188525	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T39262 WP_000621175.1 NZ_AP024723:c2183394-2183032 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T39262 NZ_AP024723:c2183394-2183032 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT39262 WP_000948331.1 NZ_AP024723:c2183561-2183391 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT39262 NZ_AP024723:c2183561-2183391 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1