Detailed information of TA system TA039249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 12503..13038
• Replicon: plasmid IN45P
• Accession: NZ_AP024719
• Organism: Methylomarinovum sp. IN45

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MIN45_RS12315
• Protein ID: WP_286294224.1
• Coordinates: 12715..13038 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MIN45_RS12310
• Protein ID: WP_286294195.1
• Coordinates: 12503..12712 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIN45_RS12295 (MIN45_PP08)	8581..9567	-	987	WP_286294220.1	PEP-CTERM sorting domain-containing protein	-
MIN45_RS12300 (MIN45_PP09)	10342..11013	+	672	WP_286294192.1	PEP-CTERM sorting domain-containing protein	-
MIN45_RS12305	11050..12171	+	1122	WP_286294194.1	PKD domain-containing protein	-
MIN45_RS12310 (MIN45_PP11)	12503..12712	+	210	WP_286294195.1	antitoxin MazE family protein	Antitoxin
MIN45_RS12315 (MIN45_PP12)	12715..13038	+	324	WP_286294224.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MIN45_RS12320 (MIN45_PP13)	13089..13322	-	234	Protein_13	type II toxin-antitoxin system HicB family antitoxin	-
MIN45_RS12325 (MIN45_PP14)	13466..14038	+	573	WP_286294197.1	recombinase family protein	-
MIN45_RS12330 (MIN45_PP15)	14121..14444	-	324	WP_286294199.1	helix-turn-helix domain-containing protein	-
MIN45_RS12335 (MIN45_PP16)	14434..14715	-	282	WP_286294225.1	BrnT family toxin	-
MIN45_RS12340 (MIN45_PP17)	14763..15032	-	270	WP_286294200.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MIN45_RS12345 (MIN45_PP18)	15016..15234	-	219	WP_286294201.1	DUF6290 family protein	-
MIN45_RS12350 (MIN45_PP19)	15608..16096	+	489	WP_286294203.1	hypothetical protein	-
MIN45_RS12355 (MIN45_PP20)	16204..16608	+	405	WP_286294204.1	hypothetical protein	-
MIN45_RS12360 (MIN45_PP21)	16733..17155	+	423	WP_286294205.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..20514	20514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (6-68)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11951.92 Da
• Isoelectric Point: 8.6049

>T39249 WP_286294224.1 NZ_AP024719:12715-13038 [Methylomarinovum sp. IN45]
VRRGDVVVVAIPGSYSKPRPAVVVQSDLFDEHPSVTILPITSHYRDAPIFRLGVFLDDRNGLEKPSQIMVDKILTVPREK
VGRVIGRLDDRTMVEINRALAVFLGMD

Nucleotide

• Length: 324 bp

>T39249 NZ_AP024719:12715-13038 [Methylomarinovum sp. IN45]
GTGCGGCGGGGGGATGTGGTGGTCGTGGCCATTCCCGGCAGCTACAGCAAGCCCAGGCCGGCGGTGGTGGTGCAGTCCGA
TCTGTTCGACGAGCATCCGTCGGTGACGATCCTGCCGATCACCAGCCATTACCGGGATGCGCCGATATTCCGGTTGGGCG
TGTTCCTAGACGACAGGAACGGCCTGGAGAAGCCGAGTCAGATCATGGTGGACAAGATTTTGACGGTTCCCAGAGAGAAG
GTGGGCCGGGTGATCGGGCGTCTCGATGACCGGACCATGGTCGAGATCAATCGAGCATTGGCGGTCTTCCTGGGGATGGA
CTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8530.55 Da
• Isoelectric Point: 6.9854

>AT39249 WP_286294195.1 NZ_AP024719:12503-12712 [Methylomarinovum sp. IN45]
MSEATERVRRHRRKLREAGLRPIQLWVPDTRNPKFREECRRQSRRLRDDPDENEMLAWIEQAADLDDWE

Nucleotide

• Length: 210 bp

>AT39249 NZ_AP024719:12503-12712 [Methylomarinovum sp. IN45]
ATGAGCGAAGCGACGGAACGGGTGCGCAGGCACCGTCGGAAATTGCGGGAAGCGGGGCTCCGGCCCATTCAGCTGTGGGT
GCCGGATACGCGCAATCCCAAGTTTCGGGAGGAATGCCGCCGGCAGTCCCGGCGGTTGCGAGACGATCCGGACGAAAATG
AGATGCTGGCGTGGATTGAGCAAGCCGCCGATCTGGACGATTGGGAGTGA