Detailed information of TA system TA039239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1338687..1339272
• Replicon: chromosome
• Accession: NZ_AP024708
• Organism: Acinetobacter sp. Tol 5

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8YNI6
• Locus tag: TOL5_RS06195
• Protein ID: WP_004829342.1
• Coordinates: 1338985..1339272 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: TOL5_RS06190
• Protein ID: WP_035300040.1
• Coordinates: 1338687..1338998 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOL5_RS06160 (TOL5_11910)	1334346..1334919	+	574	Protein_1187	nicotinate-nicotinamide nucleotide adenylyltransferase	-
TOL5_RS06165 (TOL5_11920)	1335259..1335825	+	567	WP_009584200.1	YceI family protein	-
TOL5_RS06170 (TOL5_11930)	1335876..1336793	-	918	WP_009584154.1	LysR family transcriptional regulator	-
TOL5_RS06175 (TOL5_11940)	1336891..1337496	+	606	WP_009584189.1	glutathione transferase GstA	-
TOL5_RS06180 (TOL5_11950)	1337638..1337928	-	291	WP_227510907.1	hypothetical protein	-
TOL5_RS06185 (TOL5_11960)	1338302..1338613	-	312	WP_004829346.1	hypothetical protein	-
TOL5_RS06190 (TOL5_11970)	1338687..1338998	-	312	WP_035300040.1	BrnA antitoxin family protein	Antitoxin
TOL5_RS06195 (TOL5_11980)	1338985..1339272	-	288	WP_004829342.1	BrnT family toxin	Toxin
TOL5_RS06200 (TOL5_11990)	1339411..1340301	-	891	WP_121774730.1	LysR family transcriptional regulator ArgP	-
TOL5_RS06205 (TOL5_12000)	1340479..1341078	+	600	WP_121774731.1	LysE/ArgO family amino acid transporter	-
TOL5_RS06210 (TOL5_12010)	1341229..1341690	-	462	WP_121774732.1	peroxiredoxin	-
TOL5_RS06215 (TOL5_12020)	1341694..1342395	-	702	WP_009584142.1	diphthine--ammonia ligase	-
TOL5_RS06220 (TOL5_12030)	1342459..1342869	-	411	WP_004829333.1	hypothetical protein	-
TOL5_RS06225 (TOL5_12040)	1342883..1343554	-	672	WP_009584190.1	7-cyano-7-deazaguanine synthase QueC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11281.67 Da
• Isoelectric Point: 4.6528

>T39239 WP_004829342.1 NZ_AP024708:c1339272-1338985 [Acinetobacter sp. Tol 5]
MELYFEWDEEKNQKNQKKHDVSFETASLIFEDPLRISIQDRHTDGEERWQSIGMVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T39239 NZ_AP024708:c1339272-1338985 [Acinetobacter sp. Tol 5]
ATGGAACTGTATTTCGAATGGGATGAGGAAAAGAACCAAAAGAATCAGAAAAAACATGATGTCTCTTTTGAAACAGCAAG
CCTCATTTTTGAAGATCCATTACGGATATCAATCCAAGATAGACATACTGATGGCGAAGAACGTTGGCAATCCATTGGTA
TGGTTAAAGGCGTACTAATGCTTTTAGTTGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACCAAAGCAGAGCGGGATAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 12141.78 Da
• Isoelectric Point: 8.4944

>AT39239 WP_035300040.1 NZ_AP024708:c1338998-1338687 [Acinetobacter sp. Tol 5]
MSMVRYTRKELNDNFSDKQDAEIKRLLEKGSTPDEQLDLSDIPEITEWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKEQ
GKGYQTRINKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT39239 NZ_AP024708:c1338998-1338687 [Acinetobacter sp. Tol 5]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGATAATTTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGAGAAAGGATCAACTCCTGATGAGCAATTAGACTTATCGGATATTCCTGAAATTACTGAGTGGAGTCATGCTGTTCGTC
ATGGGCAATTCTATCGCCCAGTGAAGCAACAGACTTCTGTTCGCCTTGATGCTGATGTACTTGCATGGTTAAAAGAACAA
GGTAAAGGCTATCAGACACGCATAAATAAAATTCTGCGTGAAGCTATGTTAAAAGATTTAAAAAATCATTAA

Structures

Toxin

• AlphaFold DB: N8YNI6